Fluctuations in the concentration of
glucose in the blood is more detrimental than a constantly high level of
glucose with respect to the development of cardiovascular complications associated with
diabetes mellitus (DM).
Sodium glucose cotransporter 2 (
SGLT2) inhibitors have been developed as
antidiabetic drugs with cardiovascular benefits; however, whether inhibition of SGLT1 protects the diabetic heart remains to be determined. This study investigated the role of SGLT1 in rat H9c2 cardiomyocytes subjected to fluctuating levels of
glucose and the underlying mechanisms. The results indicated that knockdown of SGLT1 restored cell proliferation and suppressed the cytotoxicity associated with fluctuating
glucose levels. Oxidative stress was induced in H9c2 cells subjected to fluctuating
glucose levels, but these changes were effectively reversed by knockdown of SGLT1, as manifested by reductions in the level of intracellular
reactive oxygen species and increased
antioxidant activity. Further study demonstrated that knockdown of SGLT1 attenuated the
mitochondrial dysfunction in H9c2 cells exposed to fluctuating
glucose levels, by restoring mitochondrial membrane potential and promoting mitochondrial fusion. In addition, knockdown of SGLT1 downregulated the expression of Bax, upregulated the expression of Bcl-2, and reduced the activation of
caspase-3 in H9c2 cells subjected to fluctuating levels of
glucose. Collectively, our results show that knockdown of SGLT1 ameliorates the apoptosis of cardiomyocyte caused by fluctuating
glucose levels via regulating oxidative stress and combatting
mitochondrial dysfunction.