Abstract | BACKGROUND: Recent studies reported abnormal alpha-synuclein deposition in biopsy-accessible sites of the peripheral nervous system in Parkinson's disease (PD). This has considerable implications for clinical diagnosis. Moreover, if deposition occurs early, it may enable tissue diagnosis of prodromal PD. OBJECTIVE: METHODS: For assay development, postmortem skin biopsies were taken from 28 patients with autopsy-confirmed Lewy body disease and 23 control subjects. Biopsies were stained for pathological alpha-synuclein in automated stainers using a novel dual-immunohistochemical assay for serine 129-phosphorylated alpha-synuclein and pan-neuronal marker protein gene product 9.5. After validation, single 3-mm punch skin biopsies were taken from the cervical 8 paravertebral area from 79 subjects (28 idiopathic rapid eye movement sleep behavior disorder, 20 PD, 10 atypical parkinsonism, and 21 control subjects). Raters blinded to clinical diagnosis assessed the biopsies. RESULTS: CONCLUSION: Even with a single 3-mm punch biopsy, there is considerable promise for using pathological alpha-synuclein deposition in skin to diagnose both clinical and prodromal PD. © 2020 International Parkinson and Movement Disorder Society.
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Authors | Ahmed Al-Qassabi, Tsu-Shuen Tsao, Adriana Racolta, Thomas Kremer, Marta Cañamero, Anton Belousov, Madison A Santana, Rachel C Beck, Hongjun Zhang, Jeffrey Meridew, Judith Pugh, Fangru Lian, Mark D Robida, Mirko Ritter, Christian Czech, Thomas G Beach, Lidija Pestic-Dragovich, Kirsten I Taylor, Wagner Zago, Lei Tang, Sebastian Dziadek, Ronald B Postuma |
Journal | Movement disorders : official journal of the Movement Disorder Society
(Mov Disord)
Vol. 36
Issue 4
Pg. 895-904
(04 2021)
ISSN: 1531-8257 [Electronic] United States |
PMID | 33232556
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © 2020 International Parkinson and Movement Disorder Society. |
Chemical References |
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Topics |
- Humans
- Lewy Body Disease
- Parkinson Disease
- REM Sleep Behavior Disorder
- Skin
- alpha-Synuclein
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