Abstract | OBJECTIVES: MATERIALS AND METHODS: To induce the memory senescence model, HUVECs, after three days incubation with high glucose, were incubated with normal glucose for another three days, and for whole six days, cells were treated with metformin (50 µM) or alpha-mangostin (1.25 µM). On the last day, cell viability by MTT assay, oxidative stress by fluorimetric assay, the number of senescent cells by SA beta-galactosidase staining kit, and secretory interleukin-6 by ELISA kit were measured. SIRT1 and P53 proteins were also evaluated by Western blotting. RESULTS: CONCLUSION: These data exhibit that alpha-mangostin, comparable to metformin, protects endothelial cells against metabolic memory-induced senescence, which is likely via SIRT1.
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Authors | Hourieh Tousian, Bibi Marjan Razavi, Hossein Hosseinzadeh |
Journal | Iranian journal of basic medical sciences
(Iran J Basic Med Sci)
Vol. 23
Issue 10
Pg. 1261-1267
(Oct 2020)
ISSN: 2008-3866 [Print] Iran |
PMID | 33149857
(Publication Type: Journal Article)
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