miR-19a promotes the metastasis and EMT through CUL5 in prostate cancer cell line PC3.

Abstract

PURPOSE:

Prostate cancer is an epithelial malignancy that occurs in the prostate and metastasis is a challenge of the treatment of prostate cancer. MicroRNA (miR)-19a was usually upregulated in several cancers at the roles of miR-19a in the metastasis in prostate cancer are still unclear.

METHODS:

A normal prostate epithelial cell line P69 and two prostate cancer cell lines PC3 and DU145 were used in this study. The mRNA levels of miR-19a and CUL5 were measured using qRT-PCR assay. Transwell and Western blot assays were conducted to calculate cell metastasis and epithelial-mesenchymal transition (EMT) properties in PC3 cells. Luciferase reporter assay was applied to validate that miR-19a targeted to CUL5.

RESULTS:

The expression of miR-19a was high in prostate cancer and its overexpression predicted poor outcome of prostate cancer patients. miR-19a regulated the expression of CUL5 by directly targeting its mRNA 3'-UTR in PC3 cells. The expression of CUL5 was lower in prostate cancer tissues and cell lines than in non-tumor tissues and normal cells. Downregulation of CUL5 predicted worse outcome of prostate cancer patients. miR-19a promoted cell migration, invasion and EMT in prostate cancer by directly binding to CUL5 mRNA 3'-UTR. CUL5 partially reversed the roles of miR-19a on the metastasis in prostate cancer.

CONCLUSION:

miR-19a promoted migratory, invasive and EMT abilities by binding to CUL5 in prostate cancer. The newly identified miR-19a/CUL5 axis provides novel insight into the pathogenesis of prostate cancer.

• Authors: Yong Wang, Jinding Hu, Guanyun Qi, Shenghui Wang, Jianjun Gao
• Journal: Journal of B.U.ON. : official journal of the Balkan Union of Oncology (J BUON) 2020 Jul-Aug Vol. 25 Issue 4 Pg. 2028-2035 ISSN: 2241-6293 [Electronic] Cyprus
• PMID: 33099949 (Publication Type: Journal Article)

Chemical References

• CUL5 protein, human
• Cullin Proteins
• MIRN19 microRNA, human
• MicroRNAs

Topics

• Cell Proliferation (physiology)
• Cullin Proteins (genetics, metabolism)
• Epithelial-Mesenchymal Transition
• Humans
• Male
• MicroRNAs (genetics, metabolism)
• Neoplasm Metastasis
• PC-3 Cells
• Prostatic Neoplasms (genetics, metabolism, pathology)
• Transfection