Structural characteristics of a mannoglucan isolated from Chinese yam and its treatment effects against gut microbiota dysbiosis and DSS-induced colitis in mice.
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| Abstract |
A water-soluble polysaccharide named CYP-1 was isolated from Chinese yam. CYP-1 was characterized as a mannoglucan having a backbone consisting predominately of 1,4-α-linked Glcp branched at O-2, O-3, and O-6 position by t-α-linked Manp with a molecular weight of 2.86 kDa. CYP-1 could inhibit the overproduction of pro-inflammatory cytokines (such as TNF-α and IL-1β) in LPS-induced RAW 264.7 cells and DSS-induced colitis mice. Oral administration of CYP-1 dramatically alleviated colonic pathological damage, suppressed the activation of colonic inflammatory signaling pathways (such as NF-κB and NLRP3 inflammasome), recovered the mRNA expression of junctional proteins (such as ZO-1, claudin-1, occludin, and connexin-43), and modulated the gut microbiota by decreasing the abundances of Alistipes, Helicobacter, and an unidentified Enterobacteriaceae, in DSS-induced colitis mice. Overall, the present study elucidated that a new polysaccharide structure CYP-1 from Chinese yam and its therapeutic potential as a prebiotic for the prevention of inflammatory bowel disease.
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| Authors | Pan Li, Nan Xiao, Liping Zeng, Jie Xiao, Jianzhao Huang, Yanan Xu, Yanlan Chen, Yunhong Ren, Bing Du |
| Journal | Carbohydrate polymers (Carbohydr Polym) Vol. 250 Pg. 116958 (Dec 15 2020) ISSN: 1879-1344 [Electronic] England |
| PMID | 33049862 (Publication Type: Journal Article) |
| Copyright | Copyright © 2020 Elsevier Ltd. All rights reserved. |
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