Abstract |
The mechanisms behind the ability of Plasmodium falciparum to evade host immune system are poorly understood and are a major roadblock in achieving malaria elimination. Here, we use integrative genomic profiling and a longitudinal pediatric cohort in Burkina Faso to demonstrate the role of post-transcriptional regulation in host immune response in malaria. We report a strong signature of miRNA expression differentiation associated with P. falciparum infection (127 out of 320 miRNAs, B-H FDR 5%) and parasitemia (72 miRNAs, B-H FDR 5%). Integrative miRNA- mRNA analysis implicates several infection-responsive miRNAs (e.g., miR-16-5p, miR-15a-5p and miR-181c-5p) promoting lymphocyte cell death. miRNA cis-eQTL analysis using whole-genome sequencing data identified 1,376 genetic variants associated with the expression of 34 miRNAs (B-H FDR 5%). We report a protective effect of rs114136945 minor allele on parasitemia mediated through miR-598-3p expression. These results highlight the impact of post-transcriptional regulation, immune cell death processes and host genetic regulatory control in malaria.
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Authors | Mame Massar Dieng, Aïssatou Diawara, Vinu Manikandan, Hala Tamim El Jarkass, Samuel Sindié Sermé, Salif Sombié, Aïssata Barry, Sam Aboubacar Coulibaly, Amidou Diarra, Nizar Drou, Marc Arnoux, Ayman Yousif, Alfred B Tiono, Sodiomon B Sirima, Issiaka Soulama, Youssef Idaghdour |
Journal | Nature communications
(Nat Commun)
Vol. 11
Issue 1
Pg. 5093
(10 09 2020)
ISSN: 2041-1723 [Electronic] England |
PMID | 33037226
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MIRN-598 microRNA, human
- MicroRNAs
- Proto-Oncogene Proteins c-bcl-2
- RNA, Messenger
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Topics |
- Burkina Faso
- Child
- Child, Preschool
- Gene Expression Regulation
- Genome, Human
- Humans
- Immune Evasion
(genetics)
- Longitudinal Studies
- Malaria, Falciparum
(genetics, immunology)
- MicroRNAs
(genetics)
- Parasitemia
(genetics, immunology)
- Plasmodium falciparum
(immunology, pathogenicity)
- Polymorphism, Single Nucleotide
- Proto-Oncogene Proteins c-bcl-2
(genetics)
- RNA, Messenger
(genetics)
- Whole Genome Sequencing
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