Collagen is a rich source of bioactive
peptides and is widely distributed in the skin and bone tissue. In this study,
collagen from Salmo salar skin was hydrolyzed with
Alcalase or Protamex followed by simulated digestion, YMC ODS-A C18 separation, and ESI-MS/MS analysis. A total of 19
peptides were identified and synthesized for investigation of their antiplatelet activities.
Hyp-Gly-Glu-
Phe-Gly (OGEFG) and
Asp-Glu-
Gly-Pro (DEGP) exhibited the most potent activity against
ADP-induced platelet aggregation among them with IC50 values of 277.17 and 290.00 μM, respectively, and inhibited the release of β-TG and
5-HT in a dose-dependent manner significantly. Single
oral administration of OGEFG and DEGP also inhibited
thrombus formation in a
ferric chloride-induced arterial
thrombosis model at a dose of 200 μmol/kg
body weight and did not prolong the bleeding time or cause an immune response in mice. Therefore, our findings indicated that
collagen peptides had a potential to be developed into an effective specific medical food in the prevention of thrombotic diseases.