Acute and subacute oral administration of mycotoxin deoxynivalenol exacerbates the pro-inflammatory and pro-pruritic responses in a mouse model of allergic dermatitis.

Abstract

Deoxynivalenol (DON) contamination in food is a public health concern; however, the effect of DON exposure on immune disorders including allergies remains unclear. The aim of this study is to elucidate the effect of oral exposure to DON on pro-inflammatory and pro-pruritic responses in a mouse model of allergic dermatitis, which was generated by topical application of toluene-2,4-diisocyanate (TDI), a hapten that induces type-2 helper T cells. To evaluate acute exposure to DON, the mice were orally administered vehicle alone, 0.1 mg/kg DON, or 0.3 mg/kg DON 48, 24, and 1 h before the final challenge with TDI. To study subacute exposure, the mice were fed DON-contaminated rodent diet (0.3 ppm) during the experimental period. After the itch behavior and ear-swelling response were monitored, the serum, auricular lymph node, and skin tissue were collected for analyzing immunocyte differentiation, cytokine determination, and histological changes. Acute oral administration of DON significantly enhanced pro-inflammatory responses including ear-swelling response, immunocyte infiltration, and cytokine productions. Histological evaluation supported the occurrence of pro-inflammatory responses. In contrast, acute DON exposure only slightly increased itch behavior. Subacute oral exposure to DON significantly up-regulated the inflammatory responses, but showed almost no effect on pruritic response. In vitro evaluation in dendritic cells and keratinocytes indicated that DON pre-exposure induced a dose-dependent significant increase in cytokine production. Our results imply that both acute and subacute exposures to DON are associated with pro-inflammatory responses in cutaneous allergy.

• Authors: Ryota Aihara, Toa Ookawara, Ai Morimoto, Naoki Iwashita, Yoshiichi Takagi, Atsushi Miyasaka, Masayo Kushiro, Shiro Miyake, Tomoki Fukuyama
• Journal: Archives of toxicology (Arch Toxicol) Vol. 94 Issue 12 Pg. 4197-4207 (12 2020) ISSN: 1432-0738 [Electronic] Germany
• PMID: 32813090 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Inflammation Mediators
• Trichothecenes
• Toluene 2,4-Diisocyanate
• deoxynivalenol

Topics

• Administration, Oral
• Animals
• Cytokines (metabolism)
• Dendritic Cells (drug effects, immunology, metabolism)
• Dermatitis, Allergic Contact (etiology, immunology, metabolism, pathology)
• Disease Models, Animal
• Female
• Humans
• Inflammation Mediators (metabolism)
• Keratinocytes (drug effects, immunology, metabolism)
• Mice, Inbred BALB C
• Pruritus (chemically induced, immunology, metabolism, pathology)
• Skin (drug effects, immunology, metabolism, pathology)
• THP-1 Cells
• Th2 Cells (drug effects, immunology, metabolism)
• Toluene 2,4-Diisocyanate
• Trichothecenes (administration & dosage, toxicity)