Beta adrenergic receptor activation inhibits oral cancer migration and invasiveness.

Abstract

OBJECTIVE:

The aim of this study was to verify β2-AR expression in oral squamous cell carcinoma cell lines (SCC-9 and SCC-25), and to investigate the role of this receptor in migration and invasion of these neoplastic cells.

DESIGN:

SCC-9 and SCC-25 cells were investigated for gene and protein expression of β2-AR. Cell migration and invasion were analyzed by wound healing assay and transwell invasion camera system. Different concentrations (0.1, 1 and 10 μM) of norepinephrine were used to stimulate, and 1 μM propranolol was used to block the beta-adrenergic receptors on cancer cells. Differences in median values of SCC-9 and SCC-25 and β2-AR protein expression were analyzed by Friedman test and in case of significant differences; pairwise comparisons were performed using Bonferroni correction.

RESULTS:

The results showed that the β2-AR gene and protein expression were observed in both oral cancer cell lines. The concentration of 10 μM of norepinephrine significantly inhibited (p ≤ 0.05) migration of SCC-9 and SCC-25 cell lines. Furthermore, there was a significant reduction (p ≤ 0.05) in the effect of norepinephrine on cell migration when the β2-AR was inhibited by propranolol. The blockade by propranolol showed a tendency to reverse the effect of norepinephrine on the invasiveness of SCC-9 and SCC-25.

CONCLUSIONS:

The use of beta-adrenergic receptor agonists could become an adjuvant therapeutic target in the treatment of this malignancy.

• Authors: Diego Mauricio Bravo-Calderón, Agnes Assao, Natália Galvão Garcia, Cláudia Malheiros Coutinho-Camillo, Martin Roffé, Janaína Naiara Germano, Denise Tostes Oliveira
• Journal: Archives of oral biology (Arch Oral Biol) Vol. 118 Pg. 104865 (Oct 2020) ISSN: 1879-1506 [Electronic] England
• PMID: 32801034 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Adrenergic beta-Antagonists
• Receptors, Adrenergic, beta
• Propranolol

Topics

• Adrenergic beta-Antagonists (pharmacology)
• Carcinoma, Squamous Cell (drug therapy, pathology)
• Cell Line, Tumor
• Humans
• Mouth Neoplasms (drug therapy, pathology)
• Neoplasm Invasiveness
• Propranolol (pharmacology)
• Receptors, Adrenergic, beta (metabolism)