MatriDerm is a
collagen-
elastin dermal template that promotes regeneration in full-thickness
wound repair. Due to its noncross-linked status,
MatriDerm biodegrades quickly in a
wound. Facilitating vascularization and dermal repair, it is desirable for
MatriDerm to remain present until the wound healing process is complete, optimizing tissue regeneration and reducing
wound contraction. The aim of this study was to investigate the effect of cross-linking
MatriDerm on its mechanical and
biological properties and to enhance its regenerative functionality.
MatriDerm was chemically cross-linked and characterized in comparison with noncross-linked
MatriDerm. Scaffold properties including surface morphology,
protein release and mechanical strength were assessed. Cell-scaffold interaction, cell proliferation and migration were examined using human dermal fibroblasts. Scaffold biodegradation and its impact on wound healing and contraction were studied in a mouse model. Results showed that cross-linked
MatriDerm displayed a small reduction in pore size, significantly less
protein loss and a threefold increase in tensile strength. A significant increase in fibroblast proliferation and migration was observed in cross-linked
MatriDerm with reduced scaffold contraction in vitro. In the mouse model, noncross-linked
MatriDerm was almost completely biodegraded after 14 days whereas cross-linked
MatriDerm remained intact. No significant difference in
wound contraction was found between scaffolds. In conclusion, cross-linked
MatriDerm showed a significant increase in stability and strength, enhancing its durability and cell-scaffold interaction. in vivo analysis showed cross-linked
MatriDerm had a reduced biodegradation rate with a similar host response. The extended structural integrity of cross-linked
MatriDerm could potentially facilitate improved skin tissue regeneration, promoting the formation of a more pliable
scar.