To enhance the qualitative bacterial biomass per unit of media and to overcome the limitations of the existing
haemorrhagic septicaemia (HS)
vaccines, a comprehensive study was undertaken encompassing the role of
iron on the bacterial biomass of Pasteurella multocida
B: 2 to
vaccine development.
Trypsin digested
hydrochloric acid-treated sheep blood (THSB) as a novel
iron rich supplement had been devised for the first time for augmenting the qualitative bacterial biomass per unit of media which was evident with growth kinetic study. The higher recovery of
iron from THSB became evident via atomic absorbance spectrophotometry. The critical level of
iron in the media as well as mode of
iron supplementation showed a major impact on the outer
membrane protein profile of P. multocida
B:2 and variation in droplet size and particle-size distribution of formulated
vaccine. Immune response study against
iron-regulated
bacterin adjuvanted with
aluminum hydroxide gel in mouse model showed that 3% THSB supplementation of
casein sucrose yeast (CSY) not only augmented the growth of P. multocida
B:2 significantly but conferred highest pre-challenged ELISA
IgG titer and protection against
pasteurellosis. Thus, THSB supplementation of CSY can resolve existing up-scaling and immunogenic potential problems of HS
vaccine production.