Polysaccharide from Ganoderma applanatum has the activities of anti-
tumor and enhancing immune function. There were no reports on antitumor effect of its intratumoral injection. In this study, the
polysaccharide was extracted from G. applanatum by water extraction and alcohol precipitation, and purified by ceramic membrane after removing
protein by Sevage method. The total
polysaccharide content from G. applanatum(
PGA)was about 63%. The combination of
PGA and
paclitaxel showed synergistic effect on cytotoxicity of 4 T1 cells at lower concentrations in vitro. In addition, the growth curve of 4 T1 cells showed that
PGA could retard the growth of 4 T1 cells gradually. The
PGA thermosensitive gel(
PGA-TG)was prepared by using
poloxamer 188 and 407. The gel temperature was 36 ℃, and the
PGA-TG could effectively slow down the release rate of
PGA in vitro. 4 T1
breast cancer-bearing mice were used as a model to evaluate the
therapeutic effect of intratumoral injection of
PGA combined with tail vein injection of nanoparticle
albumin-bound paclitaxel(nab-PTX). In high and low dose
PGA groups, each mice was given with 2.25, 1.125 mg
PGA respectively, twice in total, and the dosage of
paclitaxel was 15 mg·kg~(-1), once every 3 days, for a total of five times. The
tumor inhibition rate was 29.65% in the high dose
PGA-TG group, 58.58% in the nab-PTX group, 63.37% in low dose
PGA-TG combined with nab-PTX group, and 68.10% in high dose
PGA-TG combined with nab-PTX group respectively. The inhibitory effect in high dose
PGA-TG group combined with nab-PTX on
tumors was significantly higher than that in nab-PTX group(P<0.05). The results showed that
paclitaxel therapy combined with intratumoral injection of
PGA-TG could improve the
therapeutic effect for 4 T1 mice and reduce the side effects of
chemotherapy.