Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population.

Abstract

BACKGROUND:

Intervertebral disc degeneration (IVDD) is an age-related degenerative disease, presenting with low back pain or radicular pain. The inflammatory changes would occur in discs in the process of IVDD. Therefore, the inflammatory and anti-inflammatory cytokines, as well as their respective genes, have been proposed to play roles in pathophysiology of disease. This study has been conducted to elucidate the role of IL-2, IL-12, and IFN-γ single nucleotide polymorphisms (SNP) in this disease.

METHOD:

Seventy-six patients who were diagnosed with IVDD and 140 healthy controls who complied with eligibility criteria were included. A total volume of 5 cc peripheral blood was obtained from each participant to investigate the IL-2 + 166G/T, IL-2 -330G/T, IL-12 - 1188A/C, and IFN-γ +847A/T SNPs through PCR-SSP method.

RESULTS:

The 'TG' and 'TT' genotypes of IL-2 - 330G/T polymorphism were significantly more common among patients and healthy controls respectively. The 'GT' and 'TT' haplotypes of IL-2 (comprised of -330G/T, and + 166G/T SNPs) were also more common among patients and controls respectively.

CONCLUSION:

This study indicated the significant role of IL-2 genotypes and haplotypes in IVDD. These SNPs were differently distributed in patients and controls. Therefore, alteration in the structure of IL-2 gene could play an important role in pathophysiology of IVDD.

• Authors: Sara Hanaei, Sina Abdollahzade, Maryam Sadr, Mohammad Hossein Mirbolouk, Ehsan Fattahi, Alireza Khoshnevisan, Nima Rezaei
• Journal: BMC medical genetics (BMC Med Genet) Vol. 21 Issue 1 Pg. 143 (07 03 2020) ISSN: 1471-2350 [Electronic] England
• PMID: 32620160 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-2
• Interleukin-12
• Interferon-gamma

Topics

• Adult
• Case-Control Studies
• Disability Evaluation
• Gene Frequency (genetics)
• Genetic Association Studies
• Genetic Predisposition to Disease
• Humans
• Interferon-gamma (genetics)
• Interleukin-12 (genetics)
• Interleukin-2 (genetics)
• Intervertebral Disc Degeneration (genetics)
• Iran
• Polymorphism, Single Nucleotide
• Visual Analog Scale