LncRNA FAM83H-AS1 promotes oesophageal squamous cell carcinoma progression via miR-10a-5p/Girdin axis.

Abstract

Long non-coding RNAs (lncRNAs) have been well demonstrated to emerge as crucial regulators in cancer progression, and they can function as regulatory network based on their interactions. Although the biological functions of FAM83H-AS1 have been confirmed in various tumour progressions, the underlying molecular mechanisms of FAM83H-AS1 in oesophageal squamous cell carcinoma (ESCC) remained poorly understood. To address this, we treated human oesophageal cancer cell line Eca109 cells with TGF-β and found FAM83H-AS1 was notably overexpressed. In the present study, FAM83H-AS1 was observed to be significantly up-regulated in ESCC tissues and was associated with TNM stage, pathological differentiation and lymph node metastasis. FAM83H-AS1 reinforced oesophageal cancer cell proliferation, migration and invasion, and participated in epithelial-to-mesenchymal transition (EMT) process at mRNA and protein levels. In addition, a concordant regulation between FAM83H-AS1 and its sense strand FAM83H was detected at the transcriptional and translational levels. Furthermore, FAM83H-AS1 could act as competing endogenous RNA to affect the expression of Girdin by sponging miR-10a-5p verified by RIP and luciferase reporter assays. Consequently, the study provided a unique perspective of FAM83H-AS1 in ESCC progression, which may be considered as potential biomarker and therapeutic target for ESCC therapy.

• Authors: Bo Feng, Gaoyan Wang, Xiaoliang Liang, Zheng Wu, Xinchen Wang, Zhiming Dong, Yanli Guo, Supeng Shen, Jia Liang, Wei Guo
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 24 Issue 16 Pg. 8962-8976 (08 2020) ISSN: 1582-4934 [Electronic] England
• PMID: 32583631 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

Chemical References

• CCDC88A protein, human
• FAM83H protein, human
• MIRN10 microRNA, human
• MicroRNAs
• Microfilament Proteins
• Proteins
• RNA, Messenger
• Vesicular Transport Proteins

Topics

• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Disease Progression
• Epithelial-Mesenchymal Transition (genetics)
• Esophageal Squamous Cell Carcinoma (genetics, pathology)
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Lymphatic Metastasis (genetics, pathology)
• MicroRNAs (genetics)
• Microfilament Proteins (genetics)
• Neoplasm Invasiveness (genetics, pathology)
• Proteins (genetics)
• RNA, Messenger (genetics)
• Up-Regulation (genetics)
• Vesicular Transport Proteins (genetics)