Asiaticoside Inhibits Neuronal Apoptosis and Promotes Functional Recovery After Spinal Cord Injury in Rats.

Abstract

Spinal cord injury is a critical traumatic injury. This study aimed to investigate the possible protective effects of asiaticoside on neuronal injury in a rat model of spinal cord injury and to clarify the possible molecular mechanisms underlying these protective effects. Rats were randomly divided into the following four groups: naïve, sham, spinal cord injury (moderate mechanical compression injury model), and spinal cord injury + asiaticoside. Time to spontaneous urination after spinal cord injury was measured. The Basso-Beattie-Bresnahan scoring method, inclined plate grasp experiments, and footprint experiments were used to evaluate motor function. Neuritin and TNF-α levels in the spinal cord were detected via ELISA, and caspase-3 level was detected via Western blotting. Spinal cord morphology was assessed via hematoxylin-eosin staining. Apoptosis in the spinal cord was assessed using Fluoro-Jade B staining. Recovery times for spontaneous urination and motor function were shorter in the spinal cord injury + asiaticoside group than in the spinal cord injury group. The neuritin level was increased and TNF-α and caspase-3 levels were decreased in the spinal cord injury + asiaticoside group. Morphological integrity of neurons was better in the spinal cord injury + asiaticoside group than in the spinal cord injury group. It can thus be said that asiaticoside decreased apoptosis of spinal cord neurons. Asiaticoside exerts protective effects against spinal cord injury in rats, possibly by inhibiting the apoptosis of neurons and anti-inflammatory mechanisms.

• Authors: Lei Fan, Xiaobin Li, Tao Liu
• Journal: Journal of molecular neuroscience : MN (J Mol Neurosci) Vol. 70 Issue 12 Pg. 1988-1996 (Dec 2020) ISSN: 1559-1166 [Electronic] United States
• PMID: 32529536 (Publication Type: Journal Article)

Chemical References

• GPI-Linked Proteins
• Neuropeptides
• Neuroprotective Agents
• Nrn1 protein, rat
• Triterpenes
• Tumor Necrosis Factor-alpha
• Caspase 3
• asiaticoside

Topics

• Animals
• Apoptosis
• Caspase 3 (genetics, metabolism)
• GPI-Linked Proteins (genetics, metabolism)
• Male
• Motor Activity
• Neurons (drug effects, metabolism)
• Neuropeptides (genetics, metabolism)
• Neuroprotective Agents (pharmacology, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Spinal Cord (cytology, drug effects, metabolism)
• Spinal Cord Injuries (drug therapy)
• Triterpenes (pharmacology, therapeutic use)
• Tumor Necrosis Factor-alpha (genetics, metabolism)