Abstract | AIMS: MAIN METHODS: Human umbilical vein endothelial cells (HUVECs) were exposed to HG (30 mM) for different time periods. HG predominantly inhibited OGG1 expression in a time-dependent manner measured by western blotting, qPCR and immunofluorescence. Additionally, HUVECs were cultured with a fluorescent probe, DCFH and DHE, after being subjected to HG. Cell chemiluminescence and flow cytometry results revealed that HG caused endothelial ROS activation. KEY FINDINGS: High glucose remarkably decreased endothelial OGG1 expression. The overexpression of OGG1 significantly reversed HG-mediated PKC and NADPH oxidase activities and ROS levels. Moreover, manipulated expression of PKC significantly contacted the role of OGG1 on NADPH oxidase activation. SIGNIFICANCE: These results suggest that OGG1 downregulation promoted HG-induced endothelial ROS production and might be a potential clinical treatment target of diabetics.
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Authors | Xiangrong Xie, Yan Chen, Jichun Liu, Wenbo Zhang, Xuan Zhang, Lintao Zha, Wenjie Liu, Yang Ling, Shu Li, Shengxing Tang |
Journal | Life sciences
(Life Sci)
Vol. 256
Pg. 117886
(Sep 01 2020)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 32497631
(Publication Type: Journal Article)
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Copyright | Copyright © 2020. Published by Elsevier Inc. |
Chemical References |
- Reactive Oxygen Species
- NADPH Oxidases
- Protein Kinase C
- DNA Glycosylases
- oxoguanine glycosylase 1, human
- Glucose
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Topics |
- Animals
- DNA Glycosylases
(metabolism)
- Glucose
(toxicity)
- Human Umbilical Vein Endothelial Cells
(drug effects, metabolism)
- Humans
- Mice
- Models, Biological
- NADPH Oxidases
(metabolism)
- Protein Kinase C
(metabolism)
- Reactive Oxygen Species
(metabolism)
- Signal Transduction
(drug effects)
- Up-Regulation
(drug effects)
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