Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes.

Abstract

Ischemic heart disease is a significant cause of death worldwide. It has therefore been the subject of a tremendous amount of research, often with small-animal models such as rodents. However, the physiology of the human heart differs significantly from that of the rodent heart, underscoring the need for clinically relevant models to study heart disease. Here, we present a protocol to model ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells (hiPS-CMs) and to quantify the damage and functional impairment of the ischemic cardiomyocytes. Exposure to 2% oxygen without glucose and serum increases the percentage of injured cells, which is indicated by staining of the nucleus with propidium iodide, and decreases cellular viability. These conditions also decrease the contractility of hiPS-CMs as confirmed by displacement vector field analysis of microscopic video images. This protocol may furthermore provide a convenient method for personalized drug screening by facilitating the use of hiPS cells from individual patients. Therefore, this model of ischemic heart disease, based on iPS-CMs of human origin, can provide a useful platform for drug screening and further research on ischemic heart disease.

• Authors: Yun Liu, Yin Liang, Mengxue Wang, Chen Wang, Heng Wei, Keiji Naruse, Ken Takahashi
• Journal: Journal of visualized experiments : JoVE (J Vis Exp) Issue 159 (05 05 2020) ISSN: 1940-087X [Electronic] United States
• PMID: 32449739 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Video-Audio Media)

Topics

• Animals
• Cell Differentiation
• Cell Survival
• Disease Models, Animal
• Humans
• Induced Pluripotent Stem Cells (cytology)
• Microscopy, Video
• Myocardial Contraction
• Myocardial Ischemia (diagnostic imaging, pathology, physiopathology)
• Myocytes, Cardiac (pathology)