Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells.

Abstract

BACKGROUND:

Very few studies have identified receptor molecules for dengue virus (DENV) on neural cells. This study was designed to identify putative receptor/(s) involved in entry of DENV-3 in human neural cells of various lineages; neuronal-SH-SY5Y, astroglial-U-87 MG and microglial-CHME-3 cells.

RESULT:

Virus overlay protein binding assay, LC-MS/MS and SEQUEST identified prohibitin1/2 (PHB1/2) as interacting proteins on SH-SY5Y, CHME-3, and U-87 MG cells. Infection inhibition and siRNA assays confirmed the role of PHB1/2 in the entry of DENV-3 into SH-SY5Y and CHME-3 cells but not in U-87 MG cells. Indirect immunofluorescence and flow-cytometry demonstrated the presence of PHB1/2 on the surface of SH-SY5Y and CHME-3 cells. Co-immunoprecipitation and Western blot, as well as double labelling, reconfirmed the interaction between PHB1/2 and DENV-3 EDIII protein.

CONCLUSION:

These observations together for the first time indicate that PHB1/2 may serve as a putative receptor for DENV-3 in SH-SY5Y and CHME-3 cells. The study provided insights into DENV-3 and neural cell interactions.

• Authors: Amita Sharma, Ravi Vasanthapuram, Manjunatha M Venkataswamy, Anita Desai
• Journal: Journal of biomedical science (J Biomed Sci) Vol. 27 Issue 1 Pg. 55 (Apr 19 2020) ISSN: 1423-0127 [Electronic] England
• PMID: 32306962 (Publication Type: Journal Article)

Chemical References

• Membrane Proteins
• PHB protein, human
• Prohibitins
• Receptors, Virus
• Repressor Proteins

Topics

• Astrocytes (metabolism)
• Cell Line
• Cell Line, Tumor
• Dengue
• Dengue Virus (physiology)
• Humans
• Membrane Proteins (genetics, metabolism)
• Microglia (metabolism)
• Neuroblastoma
• Prohibitins
• Receptors, Virus (genetics, metabolism)
• Repressor Proteins (genetics, metabolism)