Abstract |
Traumatic brain injury (TBI) dysregulates microRNAs, which are the master regulators of gene expression. Here we investigated the changes in a brain-enriched miR-124-3p, which is known to associate with major post-injury pathologies, such as neuroinflammation. RT-qPCR of the rat tissue sampled at 7 d and 3 months in the perilesional cortex adjacent to the necrotic lesion core (aPeCx) revealed downregulation of miR-124-3p at 7 d (fold-change (FC) 0.13, p < 0.05 compared with control) and 3 months (FC 0.40, p < 0.05) post-TBI. In situ hybridization confirmed the downregulation of miR-124-3p at 7 d and 3 months post-TBI in the aPeCx (both p < 0.01). RT-qPCR confirmed the upregulation of the miR-124-3p target Stat3 in the aPeCx at 7 d post-TBI (7-fold, p < 0.05). mRNA-Seq revealed 312 downregulated and 311 upregulated miR-124 targets (p < 0.05). To investigate whether experimental findings translated to humans, we performed in situ hybridization of miR-124-3p in temporal lobe autopsy samples of TBI patients. Our data revealed downregulation of miR-124-3p in individual neurons of cortical layer III. These findings indicate a persistent downregulation of miR-124-3p in the perilesional cortex that might contribute to post-injury neurodegeneration and inflammation.
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Authors | Niina Vuokila, Eleonora Aronica, Anatoly Korotkov, Erwin Alexander van Vliet, Salma Nuzhat, Noora Puhakka, Asla Pitkänen |
Journal | International journal of molecular sciences
(Int J Mol Sci)
Vol. 21
Issue 7
(Mar 31 2020)
ISSN: 1422-0067 [Electronic] Switzerland |
PMID | 32244461
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- MIRN1243 microRNA, human
- MicroRNAs
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Topics |
- Animals
- Biomarkers
- Brain
(metabolism)
- Brain Injuries, Traumatic
(genetics, metabolism, pathology)
- Cerebellar Cortex
- Computational Biology
- Down-Regulation
- Female
- Gene Expression Profiling
- Humans
- Male
- MicroRNAs
(genetics, metabolism)
- Rats
- Rats, Sprague-Dawley
- Real-Time Polymerase Chain Reaction
- Transcriptome
- Up-Regulation
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