SmartPAN: A novel polysaccharide-microsphere-based surgical indicator of pancreatic leakage.

Abstract

Postoperative pancreatic fistula is a major surgical complication that can follow pancreatic resection. Postoperative pancreatic fistula can develop as a consequence of leaking pancreatic fluid, which calls for an intraoperative indicator of leakage. But suitable indicators of pancreatic leakage have yet to be found. This study details the evidence-based development and early efficacy assessments of a novel pancreatic leakage indicator (SmartPAN), following the IDEAL framework of product development. We developed 41 SmartPAN prototypes by combining indicators of pancreatic fluid with a polysaccharide-microsphere matrix. The prototypes were assessed in vitro using porcine (Sus scrofa domesticus) pancreatic tissue and ex vivo with human pancreatic fluid. From these initial tests, we chose a hydrogel-based compound that uses the pH indicator bromothymol blue to detect alkali pancreatic fluid. This prototype was then assessed in vivo for usability, effectiveness and reliability using a porcine model. Treatment groups were defined by SmartPAN-reaction at initial pancreatic resection: indicator-positive or negative. Indicator-positive individuals randomly received either targeted closure of leakage sites or no further closure. We assessed SmartPAN's reliability and effectiveness by monitoring abdominal drainage for amylase and with relaparotomy after 48 h. SmartPAN responses were consistent between both surgical procedures and conformed to amylase measurements. In conclusion, we have developed the first surgery-ready indicator for predicting the occurrence of pancreatic leakage during pancreatic resection. SmartPAN can enable targeted prophylactic closure in a simple and reliable way, and thus may reduce the impact of postoperative pancreatic fistula by guiding peri- and post-operative management.

• Authors: Thomas Moritz Pausch, Clara Mitzscherling, Sepehr Abbasi, Jiaqu Cui, Xinchun Liu, Ophelia Aubert, Melanie Weissenberger, Henrik Johansson, Peter Schuisky, Christopher Büsch, Thomas Bruckner, Mohammad Golriz, Arianeb Mehrabi, Thilo Hackert
• Journal: Journal of biomaterials applications (J Biomater Appl) Vol. 35 Issue 1 Pg. 123-134 (07 2020) ISSN: 1530-8022 [Electronic] England
• PMID: 32183581 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Indicators and Reagents
• Polysaccharides
• Smart Materials

Topics

• Animals
• Humans
• Indicators and Reagents
• Pancreas (surgery)
• Pancreatic Fistula (diagnosis)
• Polysaccharides (chemistry)
• Postoperative Complications (diagnosis)
• Smart Materials (chemistry)
• Swine