Ginsenoside Rg2 Ameliorates High-Fat Diet-Induced Metabolic Disease through SIRT1.

Abstract

Ginsenoside Rg2 has been previously reported to reduce glucose production and adipogenesis in adipose tissue. However, the effects of ginsenosides Rg2 on hepatic lipid metabolism remain vacant. In this study, we found that ginsenoside Rg2 treatment significantly attenuated oleic acid and palmitic acid (OA&PA)-induced intracellular lipid deposition and oxidative stress in mouse primary hepatocytes. C57BL/6J mice that are fed with a high-fat diet (HFD) and treated with ginsenosides Rg2 displayed decreased body weight, reversed hepatic steatosis, and improved glucose tolerance and insulin sensitivity. Ginsenoside Rg2 administration significantly ameliorated HFD-induced hepatic oxidative stress and apoptosis. Moreover, Ginsenoside Rg2 had a good affinity with Sirtuin1 (SIRT1) and regulated its expression in vivo and in vitro. Deficiency of SIRT1 eliminated the therapeutic effect of ginsenoside Rg2 on lipid accumulation and overproduction of reactive oxygen species (ROS) in OA&PA-induced mice primary hepatocytes. Ginsenoside Rg2 treatment failed to alter the lipid and glucose disorder in hepatic SIRT1 deficient mice feeding on HFD. SIRT1 deficiency dissolves the therapeutic effect of ginsenoside Rg2 on oxidative stress and hepatocyte apoptosis induced by HFD. In summary, ginsenoside Rg2 plays a therapeutic role in HFD-induced hepatosteatosis of mice by decreasing the lipogenesis process and improving antioxidant capacity in an SIRT1-dependent manner.

• Authors: Bo Cheng, Weihang Gao, Xiaojie Wu, Mingxuan Zheng, Yuanyuan Yu, Chunhui Song, Wei Miao, Zehong Yang, Yuqing He, Changhui Liu, Wangyin Yang, Xiaoying Yang, Yanwu Li, Fang Zhang, Yong Gao
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 68 Issue 14 Pg. 4215-4226 (Apr 08 2020) ISSN: 1520-5118 [Electronic] United States
• PMID: 32181656 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Blood Glucose
• Ginsenosides
• Oleic Acid
• Palmitic Acid
• ginsenoside Rg2
• Sirtuin 1

Topics

• Animals
• Antioxidants (metabolism)
• Apoptosis (drug effects)
• Blood Glucose (metabolism)
• Body Weight (drug effects)
• Diet, High-Fat (adverse effects)
• Gene Expression Regulation (drug effects)
• Ginsenosides (pharmacology)
• Insulin Resistance
• Lipid Metabolism (drug effects)
• Lipogenesis (drug effects)
• Liver
• Mice, Inbred C57BL
• Non-alcoholic Fatty Liver Disease (chemically induced, drug therapy)
• Oleic Acid (metabolism)
• Oxidative Stress (drug effects)
• Palmitic Acid (metabolism)
• Sirtuin 1 (metabolism)