Organic Spherical Nucleic Acids for the Transport of a NIR-II-Emitting Dye Across the Blood-Brain Barrier.

Abstract

DNA nanotechnology plays an increasingly important role in the biomedical field; however, its application in the design of organic nanomaterials is underexplored. Herein, we report the use of DNA nanotechnology to transport a NIR-II-emitting nanofluorophore across the blood-brain barrier (BBB), facilitating non-invasive imaging of brain tumors. Specifically, the DNA block copolymer, PS-b-DNA, is synthesized through a solid-phase click reaction. We demonstrate that its self-assembled structure shows exceptional cluster effects, among which BBB-crossing is the most notable. Therefore, PS-b-DNA is utilized as an amphiphilic matrix to fabricate a NIR-II nanofluorephore, which is applied in in vivo bioimaging. Accordingly, the NIR-II fluorescence signal of the DNA-based nanofluorophore localized at a glioblastoma is 3.8-fold higher than the NIR-II fluorescence signal of the PEG-based counterpart. The notably increased imaging resolution will significantly benefit the further diagnosis and therapy of brain tumors.

• Authors: Fan Xiao, Lin Lin, Zhicong Chao, Chen Shao, Zhe Chen, Zixiang Wei, Jingxiong Lu, Yishun Huang, Lanqing Li, Quan Liu, Yongye Liang, Leilei Tian
• Journal: Angewandte Chemie (International ed. in English) (Angew Chem Int Ed Engl) Vol. 59 Issue 24 Pg. 9702-9710 (06 08 2020) ISSN: 1521-3773 [Electronic] Germany
• PMID: 32159271 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Coloring Agents
• DNA

Topics

• Biological Transport
• Blood-Brain Barrier (metabolism)
• Cell Line
• Coloring Agents (metabolism)
• DNA (chemistry, metabolism)
• Humans
• Infrared Rays
• Molecular Imaging