Abstract | BACKGROUND: Cytology specimens are the main samples used for the diagnosis of advanced lung cancer. The objective of our study was to assess anaplastic lymphoma kinase (ALK) and c-ros oncogene 1 receptor tyrosine kinase (ROS1) genes by an amplification refractory mutation system (ARMS)-polymerase chain reaction (PCR) using cytology specimens and to then evaluate the mutation frequency of ALK and ROS1 in non-small-cell lung cancer (NSCLC) patients. METHODS: A large cohort that consisted of 8180 NSCLC patients who were genetically tested using cytology samples or formalin-fixed and paraffin-embedded (FFPE) samples ( tumor tissue or biopsy) from January 2015 to December 2018 were screened. The gene rearrangement ratio and clinical characteristics of the two sample groups were analyzed by SPSS software. RESULTS: In our hospital, cytology specimens are the main resource used for gene testing in NSCLC. In most cases, an abundant quantity of nucleic acid was extracted from the residual liquid-based cell pellet for testing the ALK and ROS1 genes. In certain cases, when the residual cell pellet was insufficient for the gene testing, the cell block and liquid-based cell smear served as alternative options. In addition, we retrospectively analyzed our previous data, and the mutation ratio of the ALK/ROS1 rearrangements obtained by using the cytology samples (4.98%/1.80%) and the FFPE samples (6.06%/1.62%) was almost the same (P-value = .09/.634). CONCLUSIONS: This study demonstrated that AMRS-PCR method can effectively identify ALK and ROS1 gene rearrangements and cytology specimens might be an excellent source for routine molecular testing in patients with advanced NSCLC.
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Authors | Ziyang Cao, Wei Wu, Wei Zhang, Zhixin Li, Caixia Gao, Yan Huang, Liping Zhang |
Journal | Diagnostic cytopathology
(Diagn Cytopathol)
Vol. 48
Issue 6
Pg. 524-530
(Jun 2020)
ISSN: 1097-0339 [Electronic] United States |
PMID | 32150350
(Publication Type: Journal Article)
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Copyright | © 2020 Wiley Periodicals, Inc. |
Chemical References |
- Proto-Oncogene Proteins
- ALK protein, human
- Anaplastic Lymphoma Kinase
- Protein-Tyrosine Kinases
- ROS1 protein, human
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Topics |
- Aged
- Anaplastic Lymphoma Kinase
(genetics)
- Carcinoma, Non-Small-Cell Lung
(genetics)
- Cytodiagnosis
(methods)
- DNA Mutational Analysis
(methods)
- Female
- Humans
- Lung Neoplasms
(genetics)
- Male
- Middle Aged
- Polymerase Chain Reaction
(methods)
- Protein-Tyrosine Kinases
(genetics)
- Proto-Oncogene Proteins
(genetics)
- Retrospective Studies
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