Abstract | OBJECTIVE: METHODS: Forty SD rats were randomly divided into 5 groups, namely sham bone cancer, bone cancer pain, morphine tolerance, bone-edge EA, and sham EA (n= 8 rats in each group). The bone cancer with morphine tolerance model was established by intramedullary injection of MRMT-1 cells into the tibial cavity, and then intraperitoneal injection of morphine hydrochloride injection. After successful establishment of morphine tolerance model, the bone-edge EA (2 Hz/100 Hz,0.5-1.5 mA) was applied to bilateral "Zusanli" (ST36) and "Kunlun" (BL60) for 30 min, once a day for 7 days, after inserting the needle-tip to the tibial bone surface. The ipsilateral mechanical paw withdrawal thresholds (PWTs) were detected dynamically. The expression levels of GRK5, β- arrestin 2, PKCα and p-PKCα in the LC area were measured by Western blot. RESULTS: The PWTs of bone cancer pain rats were decreased on day 10 after inoculation of cancer cells (P<0.01). After i.p. of morphine for 11 days, no analgesic effect and pain tolerance appeared (P>0.05). The PWTs were significantly increased in the bone-edge EA intervention group (P<0.01), not in the sham EA group (P>0.05). In comparison with the sham bone cancer group, the expression of GRK5 protein in morphine tolerance group was significantly decreased (P<0.01); compared with morphine tolerance group, the expression of GRK5 protein in bone-edge EA group was increased(P<0.01). In comparison with the sham bone cancer group, the expression of β- arrestin 2 and p-PKCα in bone cancer group significantly increased (P<0.01). After the intervention, the increased β- arrestin 2 and p-PKCα expressions were reversed in the bone-edge EA group (P<0.01); compared with morphine tolerance group and sham EA group, the expression of PKCα protein was decreased(P<0.01). CONCLUSION:
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Authors | Jun Ying Du, Feng Chen, Bin Jiang, Tao Fang Fu, Jian Qiao Fang, Yi Liang |
Journal | Zhen ci yan jiu = Acupuncture research
(Zhen Ci Yan Jiu)
Vol. 45
Issue 2
Pg. 87-92
(Feb 25 2020)
ISSN: 1000-0607 [Print] China |
PMID | 32144916
(Publication Type: Journal Article)
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Chemical References |
- beta-Arrestin 2
- Morphine
- Protein Kinase C-alpha
- G-Protein-Coupled Receptor Kinase 5
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Topics |
- Acupuncture Points
- Animals
- Bone Neoplasms
- Cancer Pain
- Electroacupuncture
- G-Protein-Coupled Receptor Kinase 5
- Locus Coeruleus
- Morphine
- Protein Kinase C-alpha
- Rats
- Rats, Sprague-Dawley
- beta-Arrestin 2
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