Dihydrocapsaicin-induced angiogenesis and improved functional recovery after cerebral ischemia and reperfusion in a rat model.

Abstract

This study investigated the long-term effects of dihydrocapsaicin (DHC)-induced angiogenesis and improved functional outcomes in cerebral ischemia and reperfusion (I/R) rats. Middle cerebral artery occlusion was induced in I/R rats for 2 h, followed by reperfusion. The animals were divided into three groups: sham, I/R + vehicle, and I/R + DHC (10 mg/kg body weight). Fourteen days after I/R injury, the DHC-treated I/R rats had decreased neurological deficit scores, infarct volume, and brain morphology changes. DHC-induced angiogenesis significantly increased the expression of angiogenic factor proteins, such as hypoxia inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF), and matrix metalloprotease 9 (MMP-9), at 3 d and 14 d following I/R and also increased the expression of angiogenic inhibitors, such as angiopoietin 1 (Ang-1) and its receptor tyrosine kinase (Tie-2), at 14 d following reperfusion. DHC-mediated angiogenesis was confirmed by a significant increase in positive BrdU labeling that co-localized with the von Willebrand factor (an endothelial cell marker) at 14 d after I/R. Furthermore, rotarod and pole tests demonstrated that DHC promoted functional recovery when compared with the vehicle group. Thus, the results reveal that DHC mediates angiogenesis and functional recovery after an ischemic stroke.

• Authors: Adchara Janyou, Piyawadee Wicha, Vatcharee Seechamnanturakit, Kanokkan Bumroongkit, Chainarong Tocharus, Apichart Suksamrarn, Jiraporn Tocharus
• Journal: Journal of pharmacological sciences (J Pharmacol Sci) Vol. 143 Issue 1 Pg. 9-16 (May 2020) ISSN: 1347-8648 [Electronic] Japan
• PMID: 32107104 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 The Authors. Production and hosting by Elsevier B.V. All rights reserved.

Chemical References

• Angiogenesis Inducing Agents
• Hif1a protein, rat
• Hypoxia-Inducible Factor 1, alpha Subunit
• Vascular Endothelial Growth Factor A
• vascular endothelial growth factor A, rat
• Matrix Metalloproteinase 9
• Capsaicin
• dihydrocapsaicin

Topics

• Angiogenesis Inducing Agents
• Animals
• Brain (blood supply, metabolism, pathology)
• Brain Ischemia (drug therapy, pathology)
• Capsaicin (analogs & derivatives, pharmacology, therapeutic use)
• Disease Models, Animal
• Gene Expression (drug effects)
• Hypoxia-Inducible Factor 1, alpha Subunit (genetics, metabolism)
• Male
• Matrix Metalloproteinase 9 (genetics, metabolism)
• Neovascularization, Physiologic (drug effects, genetics)
• Rats, Wistar
• Recovery of Function (drug effects)
• Reperfusion Injury (drug therapy, pathology)
• Vascular Endothelial Growth Factor A (genetics, metabolism)