Deficient of LRRC8A attenuates hypoxia-induced necrosis in 3T3-L1 cells.

Abstract

Under acute hypoxia, multiple ion channels on the cell membrane are activated, causing cell swelling and eventually necrosis. LRRC8A is an indispensable protein of the volume-regulated anion channel (VRAC), which participates in swelling and the acceleration of cell necrosis. In this study, we revealed a dynamic change in the expression level of the LRRC8 family during hypoxia in 3T3-L1 cells. The disruption of LRRC8A in 3T3-L1 cells was also associated with a significant anti-necrotic phenotype upon hypoxia accompanied by the reduced expression of necrosis-related genes. In vivo, differential expression of LRRC8 family members was also identified between high-altitude pigs and their low-altitude relatives. Taken these findings together, this study demonstrates the involvement of LRRC8A in hypoxia-induced cell necrosis.

• Authors: Funong Luo, Keren Long, Xiaokai Li, Miaomiao Mai, Zhining Zhong, Side Li, Penghao Li, Shuling Zhou, Tinghuan Zhang, Xi Long, Long Jin, Mingzhou Li
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 84 Issue 6 Pg. 1139-1145 (Jun 2020) ISSN: 1347-6947 [Electronic] England
• PMID: 32079488 (Publication Type: Journal Article)

Chemical References

• LRRC8A protein, mouse
• Membrane Proteins

Topics

• 3T3-L1 Cells
• Animals
• CRISPR-Cas Systems
• Cell Hypoxia (genetics)
• Cell Survival (genetics)
• Female
• Gene Expression
• Gene Knockout Techniques
• Hypoxia (genetics)
• Membrane Proteins (genetics, metabolism)
• Mice
• Myoblasts, Cardiac (metabolism)
• Necrosis (genetics)
• Rats
• Respiratory Mucosa (metabolism)
• Swine
• Transfection