Cardiac complex II activity is enhanced by fat and mediates greater mitochondrial oxygen consumption following hypoxic re-oxygenation.

Abstract

Recent evidence suggests that mitochondrial complex II is an essential mediator of myocardial ischemia-reperfusion injury. The present study aimed to investigate the effects of fatty acid supplementation or high-fat diet (HFD) on cardiac mitochondrial activity. The changes of complex I and complex II activities and mitochondrial oxygen consumption rate (OCR) following hypoxia and re-oxygenation under these conditions were studied. Our results have shown that OCR (mitochondrial activity) was significantly increased with palmitoylcarnitine supplementation in mitochondria-enriched fraction from C57BL/6 mice hearts. Mitochondrial complex I activity was unaffected by palmitoylcarnitine but complex II activity was enhanced. Re-oxygenation following 30-min hypoxia transiently increased OCR but such an effect on OCR was abolished by complex II inhibitor, malonate, but not by complex I inhibitor, rotenone, despite that complex I activity was significantly increased with re-oxygenation following hypoxia in the presence of palmitoylcarnitine. Furthermore, OCR and complex II activity were significantly increased in the mitochondria from high-fat diet mice heart compared with those of normal or low-fat diet mice. Re-oxygenation to mitochondria following 30-min hypoxia increased OCR in all three groups but significantly more in HFD. Malonate abolished re-oxygenation-induced OCR increment in all groups. Our results indicate that complex II activity and OCR are enhanced with palmitoylcarnitine or in HFD mice heart. Although re-oxygenation following hypoxia enhanced complex II and complex I activities, complex II plays an important role in increasing mitochondrial activity, which may be instrumental in myocardial injury following ischemic reperfusion.

• Authors: Shi Chao Zhu, Chen Chen, Yu Na Wu, Majid Ahmed, Ashraf Kitmitto, Adam S Greenstein, Sung Joon Kim, Yong Feng Shao, Yin Hua Zhang
• Journal: Pflugers Archiv : European journal of physiology (Pflugers Arch) Vol. 472 Issue 3 Pg. 367-374 (03 2020) ISSN: 1432-2013 [Electronic] Germany
• PMID: 32078030 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fats
• Electron Transport Complex II
• Electron Transport Complex I

Topics

• Animals
• Diet, High-Fat
• Electron Transport Complex I (metabolism)
• Electron Transport Complex II (metabolism)
• Fats (metabolism)
• Heart (physiology)
• Hypoxia (metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Mitochondria (metabolism)
• Myocardial Reperfusion Injury (metabolism)
• Oxidation-Reduction
• Oxygen Consumption (physiology)