Abstract |
Arthrospira platensis, a blue-green alga, is a popular nutraceutical substance having potent antioxidant properties with potential anti-carcinogenic activities. The aim of our study was to assess the possible anti-angiogenic effects of A platensis in an experimental model of pancreatic cancer. The effects of an A platensis extract were investigated on human pancreatic cancer cells (PA-TU-8902) and immortalized endothelial-like cells (Ea.hy926). PA-TU-8902 pancreatic tumours xenografted to athymic mice were also examined. In vitro migration and invasiveness assays were performed on the tested cells. Multiple angiogenic factors and signalling pathways were analysed in the epithelial, endothelial and cancer cells, and tumour tissue. The A platensis extract exerted inhibitory effects on both migration and invasion of pancreatic cancer as well as endothelial-like cells. Tumours of mice treated with A platensis exhibited much lesser degrees of vascularization as measured by CD31 immunostaining (P = .004). Surprisingly, the VEGF-A mRNA and protein expressions were up-regulated in pancreatic cancer cells. A platensis inhibited ERK activation upstream of Raf and suppressed the expression of ERK-regulated proteins. Treatment of pancreatic cancer with A platensis was associated with suppressive effects on migration and invasiveness with various anti-angiogenic features, which might account for the anticancer effects of this blue-green alga.
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Authors | Ivana Marková, Renata Koníčková, Kateřina Vaňková, Martin Leníček, Michal Kolář, Hynek Strnad, Miluše Hradilová, Jana Šáchová, Jan Rasl, Zuzana Klímová, Tomáš Vomastek, Ivana Němečková, Petr Nachtigal, Libor Vítek |
Journal | Journal of cellular and molecular medicine
(J Cell Mol Med)
Vol. 24
Issue 4
Pg. 2402-2415
(02 2020)
ISSN: 1582-4934 [Electronic] England |
PMID | 31957261
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. |
Chemical References |
- Angiogenesis Inhibitors
- Antineoplastic Agents
- Antioxidants
|
Topics |
- Angiogenesis Inhibitors
(pharmacology)
- Animals
- Antineoplastic Agents
(pharmacology)
- Antioxidants
(pharmacology)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Endothelial Cells
(drug effects)
- Humans
- Mice
- Mice, Nude
- Neovascularization, Pathologic
(drug therapy)
- Pancreatic Neoplasms
(drug therapy)
- Signal Transduction
(drug effects)
- Spirulina
(chemistry)
- Up-Regulation
(drug effects)
- Pancreatic Neoplasms
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