Patterns of protein expression in human head and neck cancer cell lines differ after proton vs photon radiotherapy.

Abstract

BACKGROUND:

Proton radiotherapy (PRT) may be a less toxic alternative to photon radiotherapy (XRT) for patients with head and neck squamous cell carcinoma (HNSCC). However, the molecular responses of HNSCC cells to PRT vs XRT are unclear.

METHODS:

Proteomics analyses of protein expression profiles by reverse-phase protein arrays were done for two human papillomavirus [HPV]-negative and two HPV+ cell lines. Expression patterns of 175 proteins involved in several signaling pathways were tested.

RESULTS:

Compared with PRT, XRT tended to induce lower expression of DNA damage repair-and cell cycle arrest-related proteins and higher expression of cell survival- and proliferation-related proteins.

CONCLUSIONS:

Under these experimental conditions, PRT and XRT induced different protein expression and activation profiles. Further preclinical verification is needed, as are studies of tumor pathway mutations as biomarkers for choice of treatment or as radiosensitization targets to improve the response of HNSCC to PRT or XRT.

• Authors: Li Wang, Liuqing Yang, Shichao Han, Jinming Zhu, Yuting Li, Zeming Wang, You-Hong Fan, Eric Lin, Ruiping Zhang, Narayan Sahoo, Yupeng Li, Xiaodong Zhang, Xiaochun Wang, Tengfei Li, Xiaorong R Zhu, Hongtu Zhu, John V Heymach, Jeffrey N Myers, Steven J Frank
• Journal: Head & neck (Head Neck) Vol. 42 Issue 2 Pg. 289-301 (02 2020) ISSN: 1097-0347 [Electronic] United States
• PMID: 31710172 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 Wiley Periodicals, Inc.

Chemical References

• Protons

Topics

• Cell Line, Tumor
• DNA Repair
• Head and Neck Neoplasms (genetics, radiotherapy)
• Humans
• Papillomavirus Infections
• Proteomics
• Protons
• Squamous Cell Carcinoma of Head and Neck (genetics, radiotherapy)