Synthesis and Application of CeO2/SnS2 Heterostructures as a Highly Efficient Coreaction Accelerator in the Luminol-Dissolved O2 System for Ultrasensitive Biomarkers Immunoassay.

Abstract

Electrocheluminescence (ECL) immunoassay amplified by coreaction accelerators has experienced major breakthroughs in ultrasensitive detection of biomarkers. Herein, CeO2/SnS2 heterostructures were synthesized and applied as a novel coreaction accelerator to enhance the ECL efficiency of the luminol-dissolved O2 system for the first time. Benefiting from the well-matched lattice spacing, ultrafine CeO2 nanoparticles (NPs) were grown in situ on layered SnS2 nanosheets (NSs) with improved dispersion. CeO2/SnS2 as an electroactive substrate can remarkably accelerate the generation of abundant superoxide anion radicals (O2•-) to react with luminol anion radical (L•-), achieving about 2-fold stronger ECL intensity than that of pure CeO2 NPs. To avoid harsh chemical synthesis of conventional ECL labels and simplify the antibody conjugation process, ferritin (Ft) was served as a natural nanocarrier to immobilize luminol molecules (Lum@Ft) via a one-step linkage, whose protein nanocage can easily connect with the detection antibody. Moreover, a robust site-oriented immobilization strategy using HWRGWVC heptapeptide as specific capturer was further adopted to maintain the bioactivity of the capture antibody on the amine-functionalized CeO2/SnS2 surface, which promoted the incubation efficiency markedly. On account of this advanced sensing strategy, a brand new biosensor was constructed for the accurate detection of heart failure biomarkers, which performed with favorable linearity in the range of 0.0001-50 ng/mL and achieved the detection limit of 36 fg/mL.

• Authors: Lei Yang, Yue Jia, Dan Wu, Yong Zhang, Huangxian Ju, Yu Du, Hongmin Ma, Qin Wei
• Journal: Analytical chemistry (Anal Chem) Vol. 91 Issue 21 Pg. 14066-14073 (11 05 2019) ISSN: 1520-6882 [Electronic] United States
• PMID: 31595739 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Biomarkers
• Oligopeptides
• Sulfides
• Tin Compounds
• tin sulfide
• Cerium
• Luminol
• ceric oxide
• Ferritins
• Oxygen

Topics

• Antibodies (analysis)
• Biomarkers (analysis)
• Biosensing Techniques
• Cerium (chemistry)
• Electrochemical Techniques
• Ferritins (chemistry)
• Immunoassay
• Luminol (chemistry)
• Nanoparticles (chemistry)
• Oligopeptides (analysis)
• Oxygen (chemistry)
• Particle Size
• Sulfides (chemistry)
• Surface Properties
• Tin Compounds (chemistry)