Increased susceptibility of mice obtained from in vitro fertilization to global cerebral ischemia-reperfusion injury: possible role of hydrogen sulphide and its biosynthetic enzymes.

Abstract

Aim of the Study: This study was designed to explore the relative susceptibility of in vitro fertilization (IVF)-conceived mice to global cerebral ischemic injury with the possible role of hydrogen sulphide and enzymes responsible for its production.Materials and Methods: IVF was carried to obtain pups, which were allowed to grow to the age of eight weeks. Thereafter, male mice were subjected to 20 min of global ischemia and 24 h of reperfusion. The mice obtained from other groups including normal mating, superovulation but normal mating and normal mating but embryo implantation were also subjected to global ischemia-reperfusion (I/R) injury.Results: IVF-derived mice exhibited significant more injury in response to I/R injury in comparison to other groups assessed in terms of impairment in locomotor activity, development of motor in coordination, neurological severity score, cerebral infarction and apoptosis markers (caspase-3 activity and Bcl-2 expression). Moreover, there was a relative decrease in the brain levels of hydrogen sulphide (H2S) and its biosynthetic enzymes viz. cystathionine-β-synthase and cystathionine-γ-lyase. Interestingly, the levels of H2S and cystathionine-γ-lyase were significantly low in IVF-derived mice in basal conditions also, i.e. before subjecting to I/R injury and these biochemical alterations were associated with the behavioural deficits in mice, even before subjecting to I/R injury.Conclusion: It is concluded that in vitro fertilization-derived mice are more susceptible to global cerebral I/R injury, which may be possibly due to decreased levels of hydrogen sulphide and its biosynthetic enzymes viz., cystathionine-β-synthase and cystathionine-γ-lyase.

• Authors: Hong Li, Lijun Liu, Minyan Dang, Wenzhi Zhang, Jie Liu
• Journal: The International journal of neuroscience (Int J Neurosci) Vol. 130 Issue 6 Pg. 533-540 (Jun 2020) ISSN: 1563-5279 [Electronic] England
• PMID: 31516045 (Publication Type: Journal Article)

Chemical References

• Hydrogen Sulfide

Topics

• Animals
• Apoptosis
• Behavior, Animal
• Brain Ischemia (etiology, metabolism)
• Female
• Fertilization in Vitro (adverse effects)
• Hydrogen Sulfide (metabolism)
• Male
• Mice, Inbred C57BL
• Reperfusion Injury (etiology, metabolism)