Abstract |
Number of ligations made in the chronic constriction injury (CCI) neuropathic pain model has raised serious concerns. We compared behavioural responses, nerve morphology and expression of pain marker, c-fos among CCI models developed with one, two, three and four ligations. The numbers of ligation(s) on sciatic nerve shows no significant difference in displaying mechanical and cold allodynia, and mechanical and thermal hyperalgesia throughout 84 days. All groups underwent similar levels of nerve degeneration post-surgery. Similar c-fos level in brain cingulate cortex, parafascicular nuclei and amygdala were observed in all CCI models compared to sham-operated group. Therefore, number of ligations does not impact intensity of pain symptoms, pathogenesis and neuronal activation. A single ligation is sufficient to develop neuropathic pain, in contrast to the established model of four ligations. This study dissects and characterises the CCI model, ascertaining a more uniform animal model to surrogate actual neuropathic pain condition.
|
Authors | Banulata Gopalsamy, Yogesvari Sambasevam, Nurul Atiqah Zulazmi, Jasmine Siew Min Chia, Ahmad Akira Omar Farouk, Mohd Roslan Sulaiman, Tengku Azam Shah Tengku Mohamad, Enoch Kumar Perimal |
Journal | Neurochemical research
(Neurochem Res)
Vol. 44
Issue 9
Pg. 2123-2138
(Sep 2019)
ISSN: 1573-6903 [Electronic] United States |
PMID | 31376053
(Publication Type: Journal Article)
|
Chemical References |
- Proto-Oncogene Proteins c-fos
|
Topics |
- Amygdala
(metabolism, pathology, physiopathology)
- Animals
- Constriction, Pathologic
(complications)
- Disease Models, Animal
- Gyrus Cinguli
(metabolism, pathology)
- Hyperalgesia
(metabolism, physiopathology)
- Intralaminar Thalamic Nuclei
(metabolism, pathology)
- Ligation
- Male
- Mice, Inbred ICR
- Neuralgia
(etiology, metabolism, pathology, physiopathology)
- Pain Measurement
- Proto-Oncogene Proteins c-fos
(metabolism)
- Sciatic Nerve
(injuries, pathology)
- Sciatic Neuropathy
(etiology, metabolism, pathology, physiopathology)
|