Abstract | BACKGROUND: MATERIALS AND METHODS: MTA2 expression in human oral tumor tissues and cell lines was measured by immunohistochemistry and Western blotting. Cell proliferation and cell cycle were analyzed using MTT assay and flow cytometry. The effects of MTA2 on oral cell migration and invasion were investigated using migration and invasion assays. The expression of MTA2, p-cofilin-1, and MTA2-induced LC3-II levels were measured using Western blotting and an immunofluorescence assay. RESULTS: Based on the human oral cancer tissue array and TCGA database, we found that MTA2 was increased in oral cancer tissues than in non- tumor oral tissues (P < .01). Moreover, MTA2 is significantly associated with tumor grade (P < .01) and the overall survival rate of patients with grade III tumor (P < .05). MTA2 expression in oral cancer cells was markedly higher than that in normal oral cells. Cell proliferation and cell cycle were not significantly changed in the cells inhibited by MTA2. MTA2 knockdown can inhibit cell migration and invasion of human oral cancer cells. Furthermore, we suggest that MTA2 inhibition enhances p- cofilin and LC3-II expression, and the knockdown of LC3-II expression in cells inhibited by MTA2 had the opposite effect. CONCLUSION: These results indicate that MTA2 may serve as a candidate prognostic biomarker and that targeting autophagy is a potential therapeutic strategy for treating human oral cancer.
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Authors | Tsai-Yi Tseng, Hui-Ling Chiou, Chiao-Wen Lin, Yong-Syuan Chen, Li-Sung Hsu, Chien-Hsing Lee, Yi-Hsien Hsieh |
Journal | Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
(J Oral Pathol Med)
Vol. 48
Issue 10
Pg. 959-966
(Nov 2019)
ISSN: 1600-0714 [Electronic] Denmark |
PMID | 31359510
(Publication Type: Journal Article)
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Copyright | © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- Cofilin 1
- MAP1LC3B protein, human
- Microtubule-Associated Proteins
- Repressor Proteins
- MTA2 protein, human
- Histone Deacetylases
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Topics |
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Cofilin 1
(genetics)
- Gene Knockdown Techniques
- Histone Deacetylases
(genetics)
- Humans
- Microtubule-Associated Proteins
(genetics)
- Mouth Neoplasms
(pathology)
- Neoplasm Metastasis
- Repressor Proteins
(genetics)
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