DNA methylation was involved in total glucosides of paeony regulating ERα for the treatment of female systemic lupus erythematosus mice.

Abstract

Total glucosides of paeony (TGP) is a bioactive compound extracted from paeony roots and has been used in therapy for autoimmune diseases. However the molecular mechanism of TGP in the therapy of autoimmune diseases remains unclear. ERα has a pro-inflammatory role in SLE disease. In this study, we found that TGP treatment significantly decreased the expression of ERα by up-regulating ERα promoter methylation levels. Further investigation revealed that treatment with TGP increased the expression of DNMT in lupus mice. We also used DNA methyltransferase inhibitors to verify whether DNA methylation was involved in these process. HE staining results showed that TGP can reduce renal injury in SLE mice. Moreover, cytokines including IFN-γ, IL6 and IL12 expression and dsDNA levels in serum were inhibited by TGP treatment. These findings indicate that TGP inhibits autoimmunity in SLE mice possibly by downregulate ERα expression, which may in turn be due to its ability to regulate the methylation status of the ERα promoter.

• Authors: Meiya Li, Apei Jiang
• Journal: Journal of pharmacological sciences (J Pharmacol Sci) Vol. 140 Issue 2 Pg. 187-192 (Jun 2019) ISSN: 1347-8648 [Electronic] Japan
• PMID: 31345653 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 The Authors. Production and hosting by Elsevier B.V. All rights reserved.

Chemical References

• Estrogen Receptor alpha
• Glucosides
• Inflammation Mediators
• Interleukin-6
• Interleukin-12
• Interferon-gamma
• DNA

Topics

• Animals
• Autoimmunity (drug effects)
• DNA (blood)
• DNA Methylation (drug effects)
• Down-Regulation (drug effects)
• Estrogen Receptor alpha (genetics, metabolism)
• Female
• Gene Expression (drug effects)
• Glucosides (isolation & purification, pharmacology)
• Inflammation Mediators (metabolism)
• Interferon-gamma (metabolism)
• Interleukin-12 (metabolism)
• Interleukin-6 (metabolism)
• Lupus Erythematosus, Systemic (drug therapy, genetics, immunology)
• Mice, Inbred MRL lpr
• Paeonia (chemistry)
• Phytotherapy
• Plant Roots (chemistry)