Oxidative stress has been generally considered as one trigger of organism imbalance, resulting in lipid peroxidation, DNA damage and
protein oxidation, which could be relieved by
antioxidant supplement or
endogenous antioxidant system. In present study, 1-monocaffeoyl
glycerol (1-MCG), an amphipathic
caffeic acid natural derivative, was enzymatically synthesized by
Lipozyme 435, and its
antioxidant profile in both lipophilic and lipophobic media was evaluated. The 1-MCG was identified by HPLC-UV, HPLC-ESI-MS, and 1 H/13 C-NMR. Subsequently,
antioxidant assays in lipophilic (DPPH assay) and lipophobic (
ABTS, ORAC, erythrocyte
hemolysis, ROS, MDA, and GPx assays) systems were explored. The better and lasting DPPH· and
ABTS+· inhibitions of 1-MCG than
caffeic acid (CA) were related to its better solubilities in
ethanol/water media and electron transfer ability. ORAC results suggested the radical scavenging activities of 1-MCG (5 to 40 µM) were higher than
Trolox. Furthermore, the effectiveness of 1-MCG against
AAPH-induced erythrocytes oxidation indicated that 1-MCG can effectively inhibit
hemolysis. ESEM was also applied to verify the
hemolysis inhibition and morphology preservation abilities of 1-MCG. Besides, results showed 1-MCG was able to prevent ROS from invasion, reduce production of MDA, up-regulated GPx activity, terminate lipid peroxidation, and maintain the integrity of the structure and function of erythrocytes. PRACTICAL APPLICATION: As an amphiphilic
caffeic acid derivative, 1-monocaffeoyl
glycerol was synthesized, purified, and identified. 1-Monocaffeoyl
glycerol could significantly eliminate radicals including DPPH·,
ABTS+· , and
AAPH in
ethanol, water, and PBS system, respectively. 1-Monocaffeoyl
glycerol could protect erythrocyte from
AAPH induced
hemolysis.