Abstract | BACKGROUND: METHODS: The expression levels of PD-L1 and HLA-DR were evaluated using immunohistochemical analyses. MHC class II-binding peptides for PD-L1 were designed based on computer algorithm analyses and added into in vitro culture of HTLs with antigen-presenting cells to evaluate their stimulatory activity. RESULTS: We found that seven of 24 cases of OSCC showed positive for both PD-L1 and HLA-DR and that PD-L1241-265 peptide efficiently activates HTLs, which showed not only cytokine production but also cytotoxicity against tumor cells in a PD-L1-dependent manner. Also, an adoptive transfer of the PD-L1-specific HTLs significantly inhibited growth of PD-L1-expressing human tumor cell lines in an immunodeficient mouse model. Importantly, T cell responses specific for the PD-L1241-265 peptide were detected in the HNSCC patients. CONCLUSIONS:
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Authors | Yui Hirata-Nozaki, Takayuki Ohkuri, Kenzo Ohara, Takumi Kumai, Marino Nagata, Shohei Harabuchi, Akemi Kosaka, Toshihiro Nagato, Kei Ishibashi, Kensuke Oikawa, Naoko Aoki, Mizuho Ohara, Yasuaki Harabuchi, Yuji Uno, Hidehiro Takei, Esteban Celis, Hiroya Kobayashi |
Journal | Journal of translational medicine
(J Transl Med)
Vol. 17
Issue 1
Pg. 207
(06 20 2019)
ISSN: 1479-5876 [Electronic] England |
PMID | 31221178
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- B7-H1 Antigen
- CD274 protein, human
- Epitopes, T-Lymphocyte
- Peptide Fragments
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Topics |
- Animals
- B7-H1 Antigen
(antagonists & inhibitors, genetics, metabolism)
- Cell Line, Tumor
- Cells, Cultured
- Cytotoxicity, Immunologic
(genetics, immunology)
- Epitopes, T-Lymphocyte
(chemistry, therapeutic use)
- Female
- Gene Knockdown Techniques
- Head and Neck Neoplasms
(immunology, pathology, therapy)
- Humans
- Immunotherapy
(methods, trends)
- Lymphocytes, Tumor-Infiltrating
(immunology, metabolism)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Peptide Fragments
(immunology, therapeutic use)
- Squamous Cell Carcinoma of Head and Neck
(immunology, metabolism, therapy)
- T-Lymphocytes, Helper-Inducer
(metabolism, physiology)
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