Abstract | OBJECTIVE: METHODS: Sprague-Dawley rats were used to establish an ARDS model, the expression of Piezo1, lung injuries, apoptosis as well as calcium influx were assessed. RESULTS: Piezo1 was expressed in type II pneumocytes as shown by immunofluorescence staining and expression was increased in the ARDS model. Knockdown of Piezo1 reduced apoptosis which was related to the elevation of Bcl-2.Calcium influx played a vital role in Piezo1-induced apoptosis. CONCLUSION: Piezo1 was expressed in type II pneumocytes. Mechanical stretch of alveoli during ARDS induced activation of the Piezo1 channel,which resulted in calcium influx. The increased intracellular Ca2+ induced the apoptosis of type II pneumocytes, which may be related to the Bcl-2 pathway.
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Authors | Guo-Peng Liang, Jing Xu, Li-Li Cao, Yi-Hua Zeng, Bai-Xu Chen, Jing Yang, Zhong-Wei Zhang, Yan Kang |
Journal | Respiratory research
(Respir Res)
Vol. 20
Issue 1
Pg. 118
(Jun 11 2019)
ISSN: 1465-993X [Electronic] England |
PMID | 31186017
(Publication Type: Journal Article)
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Chemical References |
- Membrane Proteins
- Piezo1 protein, rat
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Topics |
- A549 Cells
- Alveolar Epithelial Cells
(metabolism, pathology)
- Animals
- Apoptosis
(physiology)
- Humans
- Membrane Proteins
(biosynthesis)
- Rats
- Rats, Sprague-Dawley
- Respiratory Distress Syndrome
(metabolism, pathology)
- Stress, Mechanical
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