Analysis of miRNA-mRNA network reveals miR-140-5p as a suppressor of breast cancer glycolysis via targeting GLUT1.

Abstract

Aim: Aerobic glycolysis is characteristic of breast cancer. Comprehensive expression profiles of key proteins, their prognosis and detailed relationships between miRNAs and mRNAs remain unclear. Materials & methods: Oncomine database, Kaplan-Meier overall survival and miRNA-mRNA network analysis were performed. A key miRNA was identified and explored in vitro and in vivo. Results & conclusion: Eleven key glycolytic proteins were found with higher expression and poor prognosis: GLUT1, SLC2A5, HK1, PFKP, ALDOA, TPI1, GAPDH, PGK1, ENO1, GOT1 and GOT2. Seven miRNAs were predicted targeting 11 key glycolytic proteins: miR-140-5p, miR-3064-5p, miR-152-3p, miR-449b-5p, miR-449a, miR-194-5p and miR-34a-5p. Among them, miR-140-5p was found to be downregulated in breast cancer and directly targeted GLUT1, resulting in an antiglycolytic and antiproliferative effect.

• Authors: Yunjie He, Fei Deng, Shujie Zhao, Shanliang Zhong, Jianhua Zhao, Dandan Wang, Xiu Chen, Jian Zhang, Junchen Hou, Wei Zhang, Li Ding, Jinhai Tang, Zuomin Zhou
• Journal: Epigenomics (Epigenomics) Vol. 11 Issue 9 Pg. 1021-1036 (07 2019) ISSN: 1750-192X [Electronic] England
• PMID: 31184216 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Glucose Transporter Type 1
• MicroRNAs
• Mirn140 microRNA, human
• RNA, Messenger
• SLC2A1 protein, human

Topics

• Biomarkers, Tumor (genetics)
• Breast Neoplasms (diagnosis, genetics)
• Cell Line, Tumor
• Cell Proliferation
• Down-Regulation
• Female
• Glucose Transporter Type 1 (genetics)
• Glycolysis (genetics)
• Humans
• MicroRNAs (genetics)
• Prognosis
• RNA, Messenger (genetics)