miR-1246 as a therapeutic target in oral submucosa fibrosis pathogenesis.

Abstract

BACKGROUND/PURPOSE:

Oral submucous fibrosis (OSF) is a precancerous condition of oral cancer with a complex etiology. Our previous work has demonstrated that non-coding RNA miR-1246 contributes to the cancer stemness of oral cancer. In the current study, we sought to investigate the effect of the inhibition of miR-1246 on the oral fibrogenesis.

METHODS:

The expression levels of miR-1246 in OSF tissues and fibrotic buccal mucosal fibroblasts (fBMFs) were examined by qRT-PCR. Collagen gel contraction and migration assays were conducted to evaluate the myofibroblast activities. The relationship between miR-1246 and type I collagen was assessed and the protein expression of type I collagen was determined by Western blot.

RESULTS:

MiR-1246 expression was upregulated in both OSF specimen and fBMFs compared to the normal counterparts. Inhibition of miR-1246 successfully suppressed the myofibroblast activities, including collagen gel contractility and migration capacity. Moreover, the expression of miR-1246 was positively correlated with type I collagen and the expression of type I collagen was abrogated by repression of miR-1246.

CONCLUSION:

MiR-1246 is not only critical to the maintenance of oral stemness but also important to the activation of myofibroblasts. Our results showed that miR-1246 is positively associated with the type I collagen, which may be a downstream effector of miR-1246 and responsible for the fibrosis effect on fBMFs.

• Authors: Chia-Ming Liu, Yi-Wen Liao, Pei-Ling Hsieh, Chuan-Hang Yu, Pin Ju Chueh, Taichen Lin, Po-Yu Yang, Cheng-Chia Yu, Ming-Yung Chou
• Journal: Journal of the Formosan Medical Association = Taiwan yi zhi (J Formos Med Assoc) Vol. 118 Issue 7 Pg. 1093-1098 (Jul 2019) ISSN: 0929-6646 [Print] Singapore
• PMID: 30928188 (Publication Type: Journal Article)
• Copyright: Copyright © 2019. Published by Elsevier B.V.

Chemical References

• Collagen Type I
• MIRN1246 microRNA, human
• MicroRNAs

Topics

• Cell Transdifferentiation (genetics)
• Cells, Cultured
• Collagen Type I (metabolism)
• Humans
• MicroRNAs (genetics, metabolism)
• Mouth Mucosa (pathology)
• Myofibroblasts (metabolism)
• Oral Submucous Fibrosis (genetics, pathology)
• Precancerous Conditions (pathology)