Ectopic expression of miR-944 impairs colorectal cancer cell proliferation and invasion by targeting GATA binding protein 6.

Abstract

miR-944 is a microRNA that has been reported to play different important roles in the progression of cancer. Colorectal cancer (CRC) is a common cancer worldwide. A recent study has confirmed that miR-944 plays a tumour suppressive role in CRC. However, biological functions and the mechanism of miR-944 in CRC are poorly understood. Real-time reverse transcription polymerase chain reaction of 100 CRC tissues showed that miR-944 expression is frequently downregulated and is negatively associated with the T is the primary tumor, N is the lymph node, and M is the distant metastasis (TNM) stage (P = 0.009), depth of invasion (P = 0.001), and lymph node status (P = 0.002). Overexpression of mir-944 significantly impaired the functions of proliferation, migration and invasion in CRC cells, while these functions increased in knockdown experiments. GATA binding protein 6 (GATA6) knockdown can reverse the CRC cells functions induced by miR-944 inhibitor. Mechanistically, a Dual-Luciferase Reporter Assay showed that miR-944 is structurally combined with GATA6 and interacts with downstream proteins (CRT and p-AKT) in CRC cells. In conclusion, these findings indicated that miR-944 may be a tumour suppressor and could likely be used as a prognostic predictor and novel therapeutic target for CRC.

• Authors: Jing-Tong Tang, Jinbo Zhao, Weiwei Sheng, Jian-Ping Zhou, Qi Dong, Ming Dong
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 23 Issue 5 Pg. 3483-3494 (05 2019) ISSN: 1582-4934 [Electronic] England
• PMID: 30873717 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Chemical References

• GATA6 Transcription Factor
• GATA6 protein, human
• MIRN-944 microRNA, human
• MicroRNAs

Topics

• Adult
• Aged
• Aged, 80 and over
• Base Sequence
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Colorectal Neoplasms (genetics, pathology)
• Down-Regulation (genetics)
• Ectopic Gene Expression
• Female
• GATA6 Transcription Factor (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Neoplasm Invasiveness