Co‑expression of Lewis y antigen and CD147 in epithelial ovarian cancer is correlated with malignant progression and poor prognosis.

Abstract

CD147 is a highly glycosylated transmembrane protein expressed on the surface of tumor cells. In the present study, the expression and clinical significance of the Lewis y antigen and CD147 in epithelial ovarian cancer (EOC) were analyzed, and the function and correlation in between the expression of Lewis y and CD147 were evaluated using immunohistochemical staining, reverse transcription‑quantitative polymerase chain reaction analysis, immunocytochemical staining, immunoprecipitation and western blotting. The results showed that the expression of CD147 was higher in EOC tissues and correlated with a higher tumor burden. Lewis y and CD147 exhibited similar expression patterns and their expression was positively correlated. The results of the immunofluorescence and immunoprecipitation experiments showed that Lewis y and CD147 colocalized in the cell membrane and cytoplasm. Lewis y antigen, but not Lewis x or sialyl Lewis x, was predominantly expressed in the highly glycosylated form of CD147. These changes occurred at the post‑transcriptional level. As an important component of CD147, Lewis y promoted CD147‑mediated cell adhesion and the expression of matrix metalloproteinase 2. In conclusion, Lewis y antigen and CD147 were significantly upregulated in ovarian tumors, and the altered expression of Lewis y may cause changes in CD147. The two molecules are associated with carcinogenesis and the development of ovarian cancer, and Lewis y antigen is a component of the CD147 structure.

• Authors: Juanjuan Liu, Qi Liu, Yanyan Wang, Miao Liu, Yue Qi, Jian Gao, Bei Lin
• Journal: International journal of molecular medicine (Int J Mol Med) Vol. 43 Issue 4 Pg. 1687-1698 (Apr 2019) ISSN: 1791-244X [Electronic] Greece
• PMID: 30816446 (Publication Type: Journal Article)

Chemical References

• Collagen Type IV
• Laminin
• Lewis Blood Group Antigens
• Lewis Y antigen
• Basigin
• Fucosyltransferases
• galactoside 2-alpha-L-fucosyltransferase
• Matrix Metalloproteinase 2

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Basigin (metabolism)
• Carcinoma, Ovarian Epithelial (metabolism, pathology)
• Cell Adhesion
• Cell Line, Tumor
• Collagen Type IV (metabolism)
• Disease Progression
• Female
• Fucosyltransferases (metabolism)
• Humans
• Laminin (metabolism)
• Lewis Blood Group Antigens (metabolism)
• Matrix Metalloproteinase 2 (metabolism)
• Middle Aged
• Prognosis
• Survival Analysis
• Young Adult