α-Amylase and α-glucosidase inhibitor effects and pancreatic response to diabetes mellitus on Wistar rats of Ephedra alata areal part decoction with immunohistochemical analyses.

Abstract

Ephedra alata, known as a medicinal plant in China, was used in this study as aqueous extract from aerial parts, for diabetes mellitus treatment. This study was carried out on two parts, in vitro, we tested the effect of the studied extract on the inhibition of α-glucosidase and α-amylase activities, and in vivo on Wistar male rats receiving alloxan intraperitoneally at a rate of 125 mg/kg. Extract (100, 200, and 300 mg/kg of body weight) was administrated for 28 days by oral gavage. Blood glucose, amylase, lipase, and lipid profile level were determined. Oxidative stress was evaluated by enzymatic activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and by estimation of lipid peroxidation and protein carbonyl (PC) level. Histopathological changes in pancreas were investigated under photonic microscopy using immunohistochemical procedure. Our findings showed that aqueous extract inhibited in vitro both α-glucosidase and α-amylase activities and its use in vivo at 300 mg/kg of body weight restored pancreas weight and weight gain, ameliorated significantly (p ˂ 0.05) biochemical parameters; it prevented the increase in lipid and protein oxidation and the decrease in enzymatic and non-enzymatic defense system. Histological study of treated animals showed a comparable healed regeneration of beta cells.

• Authors: Jihene Ben Lamine, Mouhamed Ali Boujbiha, Sabra Dahane, Amal Ben Cherifa, Aida Khlifi, Hassiba Chahdoura, Mouhamed Taher Yakoubi, Salima Ferchichi, Nacer El Ayeb, Lotfi Achour
• Journal: Environmental science and pollution research international (Environ Sci Pollut Res Int) Vol. 26 Issue 10 Pg. 9739-9754 (Apr 2019) ISSN: 1614-7499 [Electronic] Germany
• PMID: 30729433 (Publication Type: Journal Article)

Chemical References

• Blood Glucose
• Glycoside Hydrolase Inhibitors
• Hypoglycemic Agents
• Plant Extracts
• Catalase
• Glutathione Peroxidase
• Superoxide Dismutase
• alpha-Amylases

Topics

• Animals
• Blood Glucose (analysis)
• Catalase (metabolism)
• China
• Diabetes Mellitus, Experimental
• Ephedra (chemistry)
• Glutathione Peroxidase (metabolism)
• Glycoside Hydrolase Inhibitors (toxicity)
• Hypoglycemic Agents
• Lipid Peroxidation (drug effects)
• Male
• Oxidative Stress (physiology)
• Pancreas (drug effects)
• Plant Extracts (toxicity)
• Rats
• Rats, Wistar
• Superoxide Dismutase (metabolism)
• Toxicity Tests
• alpha-Amylases (metabolism)