Epstein-Barr Virus Latent Membrane Protein-1 Induces the Expression of SUMO-1 and SUMO-2/3 in LMP1-positive Lymphomas and Cells.

Abstract

Epstein-Barr Virus latent membrane protein-1 (LMP1) interacts with the SUMO-conjugating enzyme Ubc9, which induces protein sumoylation and may contribute to LMP1-mediated oncogenesis. After analyzing human lymphoma tissues and EBV-positive cell lines, we now document a strong correlation between LMP1 and sumo-1/2/3 or SUMO-1/2/3 levels, and show that LMP1-induced sumo expression requires the activation of NF-κB signaling through CTAR1 and CTAR2. Together, these results point to a second mechanism by which LMP1 dysregulates sumoylation processes and adds EBV-associated lymphomas to the list of malignancies associated with increased SUMO expression.

• Authors: Sadia Salahuddin, Emma K Fath, Natalie Biel, Ashley Ray, C Randall Moss, Akash Patel, Sheetal Patel, Leslie Hilding, Matthew Varn, Tabithia Ross, Wyatt T Cramblet, Angela Lowrey, Joseph S Pagano, Julia Shackelford, Gretchen L Bentz
• Journal: Scientific reports (Sci Rep) Vol. 9 Issue 1 Pg. 208 (01 18 2019) ISSN: 2045-2322 [Electronic] England
• PMID: 30659232 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• EBV-associated membrane antigen, Epstein-Barr virus
• Membrane Proteins
• NF-kappa B
• SUMO-1 Protein
• SUMO2 protein, human
• SUMO3 protein, human
• Small Ubiquitin-Related Modifier Proteins
• Ubiquitins
• Viral Matrix Proteins
• Ubiquitin-Conjugating Enzymes
• ubiquitin-conjugating enzyme UBC9

Topics

• Cell Line
• HEK293 Cells
• Herpesvirus 4, Human (metabolism)
• Humans
• Lymphoma (metabolism)
• Membrane Proteins (metabolism)
• NF-kappa B (metabolism)
• Protein Binding
• SUMO-1 Protein (genetics, metabolism)
• Signal Transduction
• Small Ubiquitin-Related Modifier Proteins (genetics, metabolism)
• Sumoylation
• Ubiquitin-Conjugating Enzymes (metabolism)
• Ubiquitins (metabolism)
• Viral Matrix Proteins (metabolism, physiology)