MDA-19 Suppresses Progression of Melanoma Via Inhibiting the PI3K/Akt Pathway.

Abstract

OBJECTIVE:

To investigate the effect of MDA-19 on progression of melanoma, and explore the relevant mechanism.

METHODS:

The melanoma cell lines, M14 and UACC257, were treated with different concentrations of MDA-19, then CCK8, clone formation assay, Transwell and flow cytometry assays were performed to examine cell proliferation, migration, invasion and apoptosis, respectively. The expression of apoptosis-related proteins (Bcl-2, Bax and caspase 3 P17), EMT and signaling pathway-related proteins were also detected by Western blot.

RESULTS:

MDA-19 inhibited melanoma cells in a dose-dependent manner. Compared to the NC group, MDA-19 significantly inhibited cell growth capacity, migration and invasion of M14 and UACC257 cells, and accelerated cell apoptosis in a mitochondrial pathway through regulating Bcl-2/Bax and Caspase 3 in M14 and UACC257 cells. Moreover, MDA-19 was observed to up-regulate the expression of E-cad and down-regulate the expression of N-cad, Vimentin and Slug in melanoma cells in vitro. Furthermore, MDA-19 could inhibit the PI3K/Akt pathway by blocking Akt phosphorylation (p-Akt) and downstream proteins, P70 and Cyclin D1 in M14 and UACC257 cells.

CONCLUSION:

Our data demonstrate that MDA-19 could inhibit progression of melanoma by suppressing the PI3K/Akt pathway, suggesting that MDA-19 is a potential anti-cancer agent for therapy of melanoma.

• Authors: Ningning Dang, Xianguang Meng, Shanshan Ma, Qian Zhang, XiYa Sun, Jingjing Wei, Shuhong Huang
• Journal: Open medicine (Warsaw, Poland) (Open Med (Wars)) Vol. 13 Pg. 416-424 ( 2018) ISSN: 2391-5463 [Print] Poland
• PMID: 30613786 (Publication Type: Journal Article)