Kaempferol suppresses cell migration through the activation of the ERK signaling pathways in ARPE-19 cells.

Abstract

Kaempferol is a flavonoid with anticancer and anti-metastasis activity in different cancer-cell lines. However, the underlying mechanisms by which kaempferol acts on human retinal pigment epithelial (ARPE-19) cells remain unclear. In this study, we demonstrated that kaempferol inhibited migration and invasion in ARPE-19 cells at non-toxic dosages. We discovered that kaempferol obviously reduced the enzyme activity and protein expression of matrix metalloproteinase-2 by increasing the phosphorylated levels of extracellular signal-regulated kinases 1/2 (ERK1/2) signaling pathways. Additionally, ERK1/2-specific inhibitor PD98059 significantly reversed kaempferol's inhibitory effects on migration and expression of MMP-2 in ARPE-19 cells. Overall, our results are the first to demonstrate that kaempferol is capable of inhibiting cell migration by targeting ERK1/2 signaling in human retinal pigment epithelial cells.

• Authors: Hsiang-Wen Chien, Kai Wang, Yuan-Yen Chang, Yi-Hsien Hsieh, Nuo-Yi Yu, Shun-Fa Yang, Hui-Wen Lin
• Journal: Environmental toxicology (Environ Toxicol) Vol. 34 Issue 3 Pg. 312-318 (Mar 2019) ISSN: 1522-7278 [Electronic] United States
• PMID: 30499162 (Publication Type: Journal Article)
• Copyright: © 2018 Wiley Periodicals, Inc.

Chemical References

• Kaempferols
• kaempferol
• Mitogen-Activated Protein Kinase 1
• Mitogen-Activated Protein Kinase 3
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Adult
• Cell Line
• Cell Movement (drug effects)
• Epithelial Cells (cytology, drug effects, metabolism)
• Humans
• Kaempferols (pharmacology)
• MAP Kinase Signaling System (drug effects)
• Male
• Matrix Metalloproteinase 2 (genetics, metabolism)
• Matrix Metalloproteinase 9 (genetics, metabolism)
• Mitogen-Activated Protein Kinase 1 (genetics, metabolism)
• Mitogen-Activated Protein Kinase 3 (genetics, metabolism)
• Phosphorylation (drug effects)
• Retinal Pigment Epithelium (drug effects, metabolism)
• Young Adult