Loss of charge mutations in solvent exposed Lys residues of superoxide dismutase 1 do not induce inclusion formation in cultured cell models.

Abstract

Mutations in superoxide dismutase 1 (SOD1) associated with familial amyotrophic lateral sclerosis (fALS) induce the protein to misfold and aggregate. Missense mutations at more than 80 different amino acid positions have been associated with disease. How these mutations heighten the propensity of SOD1 to misfold and aggregate is unclear. With so many mutations, it is possible that more than one mechanism of aggregation may be involved. Of many possible mechanisms to explain heightened aggregation, one that has been suggested is that mutations that eliminate charged amino acids could diminish repulsive forces that would inhibit aberrant protein:protein interactions. Mutations at twenty-one charged residues in SOD1 have been associated with fALS, but of the 11 Lys residues in the protein, only 1 has been identified as mutated in ALS patients. Here, we examined whether loss of positively charged surface Lys residues in SOD1 would induce misfolding and formation of intracellular inclusions. We mutated four different Lys residues (K30, K36, K75, K91) in SOD1 that are not particularly well conserved, and expressed these variants as fusion proteins with yellow fluorescent protein (YFP) to assess inclusion formation. We also assessed whether these mutations induced binding to a conformation-restricted SOD1 antibody, designated C4F6, which recognizes non-natively folded protein. Although we observed some mutations to cause enhanced C4F6 binding, we did not observe that mutations that reduce charge at these positions caused the protein to form intracellular inclusions. Our findings may have implications for the low frequency of mutations at Lys residues SOD1 in ALS patients.

• Authors: Keith Crosby, Anthony M Crown, Brittany L Roberts, Hilda Brown, Jacob I Ayers, David R Borchelt
• Journal: PloS one (PLoS One) Vol. 13 Issue 11 Pg. e0206751 ( 2018) ISSN: 1932-6203 [Electronic] United States
• PMID: 30399166 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• Luminescent Proteins
• SOD1 protein, human
• yellow fluorescent protein, Bacteria
• Superoxide Dismutase-1

Topics

• Amino Acid Sequence
• Amyotrophic Lateral Sclerosis (genetics, metabolism)
• Animals
• Bacterial Proteins (genetics, metabolism)
• CHO Cells
• Cricetulus
• Inclusion Bodies (metabolism)
• Luminescent Proteins (genetics, metabolism)
• Mutation
• Protein Aggregation, Pathological (genetics, metabolism)
• Protein Binding
• Protein Conformation
• Protein Folding
• Superoxide Dismutase-1 (genetics, metabolism)