SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation.

Abstract

Some exciting biological questions require quantifying thousands of proteins in single cells. To achieve this goal, we develop Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS) and validate its ability to identify distinct human cancer cell types based on their proteomes. We use SCoPE-MS to quantify over a thousand proteins in differentiating mouse embryonic stem cells. The single-cell proteomes enable us to deconstruct cell populations and infer protein abundance relationships. Comparison between single-cell proteomes and transcriptomes indicates coordinated mRNA and protein covariation, yet many genes exhibit functionally concerted and distinct regulatory patterns at the mRNA and the protein level.

• Authors: Bogdan Budnik, Ezra Levy, Guillaume Harmange, Nikolai Slavov
• Journal: Genome biology (Genome Biol) Vol. 19 Issue 1 Pg. 161 (10 22 2018) ISSN: 1474-760X [Electronic] England
• PMID: 30343672 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Proteome
• RNA, Messenger

Topics

• Animals
• Cell Differentiation
• Cell Line
• Humans
• Mammals (metabolism)
• Mass Spectrometry (methods)
• Mice
• Mouse Embryonic Stem Cells (cytology, metabolism)
• Principal Component Analysis
• Proteome (metabolism)
• Proteomics (methods)
• RNA, Messenger (genetics, metabolism)
• Single-Cell Analysis