Skin
fibrosis is a chronic debilitating feature of several
skin diseases that lead to characteristic increases in dermal fibroblast proliferation and
collagen deposition through upregulation in components of the
transforming growth factor beta (TGF-B)/SMAD pathway. In contrast to ultraviolet
phototherapy, high-fluence light-emitting diode-generated red light (HF-LED-RL, 633 ± 15 nm) is a safe, economic and non-invasive
therapy with in vitro evidence that supports modulation of the key cellular characteristics involved in the pathogenesis of skin
fibrosis. Limited data exists pertaining to the effects of HF-LED-RL on human skin fibroblast
microRNA (
miRNA). Herein, we explored the effects of HF-LED-RL on fibroblast
miRNA levels using
RNA-seq and
miRNA expression analysis. Using
RNA-seq analysis we found that HF-LED-RL at 320 and 640 J/cm2 increased transcription of key
miRNA that are involved in skin
fibrosis including miRNA-29, miRNA-196a and Let-7a, and decreased transcription of miRNA-21, miRNA-23b and miRNA-31. These
microRNA findings provide insight into the molecular underpinnings of HF-LED-RL and highlight potential therapeutic targets of interest for the treatment of skin
fibrosis. Additional research on the specific molecular mechanisms underlying HF-LED-RL effects on fibroblasts may provide further mechanistic insight into this
therapy and may reveal additional future therapeutic targets for skin
fibrosis.