Effects of Ginsenoside Rb1 on Expressions of Phosphorylation Akt/Phosphorylation mTOR/Phosphorylation PTEN in Artificial Abnormal Hippocampal Microenvironment in Rats.

Abstract

Artificial abnormal microenvironment caused by microperfusion of L-glutamate (Glu) and Ca2+ in the hippocampus results in neuron damage, which is closely related to cerebral ischemia. Ginsenoside Rb1, a compound from Panax notoginseng, was previously used to counter the artificial abnormal hippocampal environment in a microperfusion model. In addition, while the Akt/mTOR/PTEN signaling pathway has been shown to mediate neuronprotection in cerebral ischemia, whether this pathway is involved in the neuroprotection of ginsenoside Rb1 is unknown. Here SH-SY5Y cells exposed to OGD/R injury in treated with LY294002, ginsenoside Rb1, ginsenoside Rb1+ LY294002. Expressions of phosphorylation (P-)Akt/P-mTOR/P-PTEN (24 h after OGD/R) were detected by Western blotting. Effects were examined via the memory function of rats (by Morris water maze test), morphological changes in pyramidal cell (by histology), and mRNA expression (by qRT-PCR) and phosphorylation (P-) (by Western blotting and immunohistochemical staining) of Akt, P-mTOR, and P-PTEN in the hippocampus. The memory deficit of rats and pyramidal cellular necrosis and apoptosis in the CA1 region of hippocampus after microperfusion of Glu and Ca2+ were dose dependently alleviated by ginsenoside Rb1.Moreover,Western blot showed that ginsenoside Rb1 increased the expressions of P-Akt, P-mTOR and reduced P-PTEN in vivo and vitro. Thus, the potent neuroprotection of ginsenoside Rb1 in artificial abnormal microenvironment is, at least partially, related to the activation of P-AKT/P-mTOR signaling pathway and inhibition of P-PTEN protein.

• Authors: Ying Guo, Li-Ping Wang, Chen Li, Yun-Xia Xiong, Yi-Tian Yan, Li-Qin Zhao, Shu-De Li, Jun Sun, Hai-Yun Luo, Cory J Xian
• Journal: Neurochemical research (Neurochem Res) Vol. 43 Issue 10 Pg. 1927-1937 (Oct 2018) ISSN: 1573-6903 [Electronic] United States
• PMID: 30167941 (Publication Type: Journal Article)

Chemical References

• Chromones
• Ginsenosides
• Morpholines
• 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
• ginsenoside Rb1
• mTOR protein, rat
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases
• PTEN Phosphohydrolase
• Pten protein, rat

Topics

• Animals
• Brain Ischemia (drug therapy, metabolism)
• Chromones (pharmacology)
• Ginsenosides (pharmacology)
• Hippocampus (drug effects, metabolism)
• Male
• Morpholines (pharmacology)
• PTEN Phosphohydrolase (drug effects, metabolism)
• Phosphorylation (drug effects)
• Proto-Oncogene Proteins c-akt (drug effects, metabolism)
• Rats, Sprague-Dawley
• Signal Transduction (drug effects)
• TOR Serine-Threonine Kinases (drug effects, metabolism)