Coxiella burnetii is a common cause of blood culture-negative
infective endocarditis (IE). Molecular detection of C burnetii
DNA in clinical specimens is a promising method of diagnosing
Q fever endocarditis. Here, we examined the diagnostic utility of
Q fever polymerase chain reaction (PCR) of
formalin-fixed heart valve tissue from patients with blood culture-negative IE who underwent heart valve surgery. Clinical and laboratory data of patients with blood culture-negative IE who underwent heart valve surgery during a 6-year period and for whom biopsy tissues were available were reviewed retrospectively. Blood culture-positive IE patients who underwent heart valve surgery within the last 3 years were used as controls. Heart valve samples were cultured and also subjected to histological examination and PCR for
Q fever,
brucellosis, and
bartonellosis. Data from 20 patients with blood culture-negative IE and 20 with blood culture-positive IE were analyzed. Eight cases of blood culture-negative IE were PCR-positive for C burnetii (40%; 95% confidence interval, 19-64). No specimen was PCR-positive for
brucellosis or
bartonellosis. Histologically, 4 of 8 specimens with a positive
Q fever PCR result were characterized by clusters of multinucleated giant cells without a
fibrin ring. None of 20 patients with blood culture-negative IE received anti-Coxiella
antibiotic therapy due to lack of clinical suspicion. Six-month mortality was higher in the
Q fever PCR-positive group than in the
Q fever PCR-negative group [38% (3/8) vs 0% (0/12), Pā=ā.049). Of the 20 patients with blood culture-positive IE, none yielded a positive
Q fever PCR result for valve tissue. Approximately 40% of patients with culture-negative IE who received heart valve surgery were PCR-positive for
Q fever; patients without clinical suspicion suffered high mortality. These data suggest that
Q fever IE in patients with culture-negative IE is often missed in routine clinical practice.